SRP40/YKR092C Summary

SRP40/YKR092C Summary

Standard Name	SRP40
Systematic Name	YKR092C
Feature Type	ORF, Verified
Description	Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 (1, 2, 3)
Name Description	Serine Rich Protein 4

Chromosomal Location	ChrXI:613885 to 612665 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SRP40 GO evidence and references


Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	nucleocytoplasmic transport (ISS)
Cellular Component
Manually curated	nucleolus (IDA)

Mutant phenotypes All SRP40 Phenotype evidence and references

Classical genetics
null	box C/D snoRNA construct derived from U14 (snR128) localization: abnormal resistance to sirolimus: increased resistance to tellurite: decreased
overexpression	vegetative growth: decreased rate
Large-scale survey
null	budding index: decreased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to camptothecin: increased resistance to bleomycin: increased resistance to cycloheximide: increased resistance to benzo[a]pyrene: decreased resistance to fenpropimorph: increased resistance to fluconazole: increased resistance to methyl methanesulfonate: increased resistance to mycophenolic acid: increased resistance to 5-fluorouracil: decreased resistance to caffeine: increased resistance to BPS: increased starvation resistance: decreased viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SRP40 Interaction evidence and references

	173 total interaction(s) for 138 unique genes/features.
Physical Interactions	Affinity Capture-MS: 27 Affinity Capture-RNA: 4 Biochemical Activity: 10 PCA: 4 Two-hybrid: 4
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 3 Negative Genetic: 64 Phenotypic Suppression: 1 Positive Genetic: 48 Synthetic Growth Defect: 5 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SRP40 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:613885 to 612665 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1221	613885..612665	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001800

References cited on this page View Complete Literature Guide for SRP40

1)	Yang Y, et al. (2000) Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140. Mol Biol Cell 11(2):567-77
2)	Ikonomova R, et al. (1997) The Srp40 protein plays a dose-sensitive role in preribosome assembly or transport and depends on its carboxy-terminal domain for proper localization to the yeast nucleoskeleton. DNA Cell Biol 16(10):1161-73
3)	Meier UT (1996) Comparison of the rat nucleolar protein nopp140 with its yeast homolog SRP40. Differential phosphorylation in vertebrates and yeast. J Biol Chem 271(32):19376-84
4)	Lalo D, et al. (1993) Interactions between three common subunits of yeast RNA polymerases I and III. Proc Natl Acad Sci U S A 90(12):5524-8