Standard Name	PRP16 1
Systematic Name	YKR086W
Alias	RNA16 2 , PRP23 3 , 4
Feature Type	ORF, Verified
Description	DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step (5, 6, 7)
Name Description	Pre-mRNA Processing 4

Chromosomal Location	ChrXI:599857 to 603072 | ORF Map | GBrowse

	View Computational GO annotations for PRP16
Molecular Function
Manually curated	RNA-dependent ATPase activity (IDA) contributes_to second spliceosomal transesterification activity (IMP)
Biological Process
Manually curated	generation of catalytic spliceosome for second transesterification step (IMP)
Cellular Component
Manually curated	U2-type catalytic step 2 spliceosome (IDA)

Classical genetics
conditional	cold sensitivity: increased
null	inviable
unspecified	unspliced pre-mRNA accumulation: increased spindle morphology: abnormal
Large-scale survey
conditional	resistance to benomyl: decreased
null	haploinsufficient inviable starvation resistance: decreased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	168 total interaction(s) for 141 unique genes/features.
Physical Interactions	Affinity Capture-MS: 37 Affinity Capture-Western: 3 Co-fractionation: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 83 Phenotypic Enhancement: 1 Positive Genetic: 12 Synthetic Growth Defect: 3 Synthetic Lethality: 21 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:599857 to 603072 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3216 599857..603072 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001794