PRP16/YKR086W Summary Help

Standard Name PRP16 1
Systematic Name YKR086W
Alias RNA16 2 , PRP23 3 , 4
Feature Type ORF, Verified
Description DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa (5, 6, 7, 8, 9)
Name Description Pre-mRNA Processing 4
Chromosomal Location
ChrXI:599857 to 603072 | ORF Map | GBrowse
Gene Ontology Annotations All PRP16 GO evidence and references
  View Computational GO annotations for PRP16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
169 total interaction(s) for 142 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 38
  • Affinity Capture-Western: 3
  • Co-fractionation: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 83
  • Phenotypic Enhancement: 1
  • Positive Genetic: 12
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 21
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 1,071
Molecular Weight (Da) 121,651
Isoelectric Point (pI) 8.25
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:599857 to 603072 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3216 599857..603072 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001794
References cited on this page View Complete Literature Guide for PRP16
1) Burgess S, et al.  (1990) A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell 60(5):705-17
2) Couto JR, et al.  (1987) A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation. Genes Dev 1(5):445-55
3) Schwer B and Guthrie C  (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349(6309):494-9
4) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
5) Wang Y, et al.  (1998) The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro. Curr Biol 8(8):441-51
6) Umen JG and Guthrie C  (1995) The second catalytic step of pre-mRNA splicing. RNA 1(9):869-85
7) Tseng CK, et al.  (2011) DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. RNA 17(1):145-54
8) Ohrt T, et al.  (2013) Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system. RNA 19(7):902-15
9) Ajmal M, et al.  (2014) A missense mutation in the splicing factor gene DHX38 is associated with early-onset retinitis pigmentosa with macular coloboma. J Med Genet 51(7):444-8