NUP133/YKR082W Summary Help

Standard Name NUP133 1
Systematic Name YKR082W
Alias RAT3 2
Feature Type ORF, Verified
Description Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133 (3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and see Summary Paragraph)
Name Description NUclear Pore
Chromosomal Location
ChrXI:592825 to 596298 | ORF Map | GBrowse
Gene Ontology Annotations All NUP133 GO evidence and references
  View Computational GO annotations for NUP133
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
537 total interaction(s) for 311 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 55
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Co-localization: 1
  • Co-purification: 2
  • FRET: 4
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 1
  • Negative Genetic: 297
  • Phenotypic Enhancement: 3
  • Positive Genetic: 52
  • Synthetic Growth Defect: 32
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 74
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,157
Molecular Weight (Da) 133,319
Isoelectric Point (pI) 4.85
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:592825 to 596298 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3474 592825..596298 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001790

NUP133 encodes a nuclear pore protein that was first identified in a screen for mutations synthetically lethal with a nup49 mutant (13, 14). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 14, 15, 16, 17). The structure of the vertebrate NPC has been studied extensively; recent reviews include 18, 19, 20, and 21. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (22, 23). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 14, 15, 24, 25, 26). NUP133 is not essential; nup133 deletion causes temperature sensitivity, altered distribution (clustering) of nuclear pore complexes, and a defect in RNA export from the nucleus (13, 2, 1, 27, 14). nup133 mutations are also synthetically lethal with mutations in several other nucleoporin genes (14).

Last updated: 1999-08-10 Contact SGD

References cited on this page View Complete Literature Guide for NUP133
1) Pemberton LF, et al.  (1995) Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc Natl Acad Sci U S A 92(4):1187-91
2) Li O, et al.  (1995) Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes. Mol Biol Cell 6(4):401-417
3) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
4) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
5) Allen NP, et al.  (2002) Deciphering networks of protein interactions at the nuclear pore complex. Mol Cell Proteomics 1(12):930-46
6) Therizols P, et al.  (2006) Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region. J Cell Biol 172(2):189-99
7) Lutzmann M, et al.  (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J 21(3):387-97
8) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
9) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
10) Sarma NJ, et al.  (2011) The nuclear pore complex mediates binding of the mig1 repressor to target promoters. PLoS One 6(11):e27117
11) Fernandez-Martinez J, et al.  (2012) Structure-function mapping of a heptameric module in the nuclear pore complex. J Cell Biol 196(4):419-34
12) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
13) Doye V, et al.  (1994) A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J 13(24):6062-75
14) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
15) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
16) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
17) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
18) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
19) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
20) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
21) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
22) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
23) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
24) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
25) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
26) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
27) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301