NUP133/YKR082W Summary Help

NUP133 BASIC INFORMATION

Standard Name NUP133
Systematic Name YKR082W
Alias RAT3
Feature Type ORF, Verified
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p (1, 2, 3 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP133 GO evidence and references
    View Computational GO annotations for NUP133
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP133 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions NUP133 All interactions details and references
207 total interaction(s) for 129 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-Western: 1
  • Co-purification: 2
  • FRET: 4
  • Reconstituted Complex: 2
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 58
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 20
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 77
  • Synthetic Rescue: 1
Sequence Information
ChrXI:592467 to 595940 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2005-12-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3474 592467..595940 2005-12-15 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001790

NUP133 RESOURCES

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  • Localization Resources
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Expression Summary histogram

ADDITIONAL INFORMATION for NUP133

SUMMARY PARAGRAPH for NUP133

NUP133 encodes a nuclear pore protein that was first identified in a screen for mutations synthetically lethal with a nup49 mutant (4, 5). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 5, 6, 7, 8). The structure of the vertebrate NPC has been studied extensively; recent reviews include 9, 10, 11, and 12. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (13, 14). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 5, 6, 15, 16, 17). NUP133 is not essential; nup133 deletion causes temperature sensitivity, altered distribution (clustering) of nuclear pore complexes, and a defect in RNA export from the nucleus (4, 18, 19, 20, 5). nup133 mutations are also synthetically lethal with mutations in several other nucleoporin genes (5).

Last updated: 1999-08-10

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP133]

1) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
2) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
3) Allen NP, et al.  (2002) Deciphering networks of protein interactions at the nuclear pore complex. Mol Cell Proteomics 1(12):930-46
4) Doye V, et al.  (1994) A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J 13(24):6062-75
5) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
6) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
8) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
9) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
10) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
11) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
12) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
13) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
14) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
15) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
16) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
17) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
18) Li O, et al.  (1995) Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes. Mol Biol Cell 6(4):401-417
19) Pemberton LF, et al.  (1995) Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc Natl Acad Sci U S A 92(4):1187-91
20) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301