RPF2/YKR081C Summary

RPF2 BASIC INFORMATION

Standard Name	RPF2
Systematic Name	YKR081C
Feature Type	ORF, Verified
Description	Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles (1, 2)

GO Annotations	All RPF2 GO evidence and references
	View Computational GO annotations for RPF2
Molecular Function
Manually curated	5S rRNA binding (IPI) 7S RNA binding (IPI) rRNA binding (IPI)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit assembly (IMP, IPI)
Cellular Component
Manually curated	nucleolus (IDA, IPI)
High-throughput	preribosome, large subunit precursor (IDA)

Mutant Phenotype	All RPF2 Phenotype details and references
Classical genetics
null	inviable
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
overexpression	vegetative growth: decreased

Interactions	RPF2 All interactions details and references
	195 total interaction(s) for 88 unique genes/features.
Physical Interactions	Affinity Capture-MS: 166 Affinity Capture-Western: 13 Co-fractionation: 1 Co-purification: 5 Far Western: 4 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1

Sequence Information

ChrXI:592182 to 591148 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-12-15 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1035	592182..591148	2005-12-15	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001789

RPF2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RPF2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPF2]

1)	Morita D, et al. (2002) Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiae. J Biol Chem 277(32):28780-6
2)	Horsey EW, et al. (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27

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