SIS2/YKR072C Summary Help

Standard Name SIS2 1, 2
Systematic Name YKR072C
Alias HAL3 3
Feature Type ORF, Verified
Description Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication (4, 5, 6, 7, 8)
Name Description SIt4 Suppressor 1
Chromosomal Location
ChrXI:578123 to 576435 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 57 cM
Gene Ontology Annotations All SIS2 GO evidence and references
  View Computational GO annotations for SIS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
146 total interaction(s) for 93 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • PCA: 8
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 10
  • Dosage Lethality: 3
  • Dosage Rescue: 31
  • Negative Genetic: 22
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 9
  • Positive Genetic: 2
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 12
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 562
Molecular Weight (Da) 62,478
Isoelectric Point (pI) 4.4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:578123 to 576435 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 57 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1689 578123..576435 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001780
References cited on this page View Complete Literature Guide for SIS2
1) Di Como CJ, et al.  (1995) Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants. Genetics 139(1):95-107
2) Sutton, A. and Arndt, K.  (1992) Personal Communication, Mortimer Map Edition 11
3) Ferrando A, et al.  (1995) Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3. Mol Cell Biol 15(10):5470-81
4) de Nadal E, et al.  (1998) The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase. Proc Natl Acad Sci U S A 95(13):7357-62
5) Yenush L, et al.  (2002) The Ppz protein phosphatases are key regulators of K+ and pH homeostasis: implications for salt tolerance, cell wall integrity and cell cycle progression. EMBO J 21(5):920-9
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Ruiz A, et al.  (2009) Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis. Nat Chem Biol 5(12):920-8
8) Olzhausen J, et al.  (2013) Molecular characterization of the heteromeric coenzyme A-synthesizing protein complex (CoA-SPC) in the yeast Saccharomyces cerevisiae. FEMS Yeast Res 13(6):565-73