BET3/YKR068C Summary Help

Standard Name BET3 1
Systematic Name YKR068C
Feature Type ORF, Verified
Description Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles (1, 2, 3, 4, 5)
Name Description Blocked Early in Transport 6
Chromosomal Location
ChrXI:570909 to 570328 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All BET3 GO evidence and references
  View Computational GO annotations for BET3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
Resources
205 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 57
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Lethality: 4
  • Dosage Rescue: 14
  • Negative Genetic: 26
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 20

Resources
Expression Summary
histogram
Resources
Length (a.a.) 193
Molecular Weight (Da) 22,129
Isoelectric Point (pI) 4.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:570909 to 570328 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..582 570909..570328 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001776
References cited on this page View Complete Literature Guide for BET3
1) Rossi G, et al.  (1995) BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs. Mol Biol Cell 6(12):1769-80
2) Sacher M, et al.  (1998) TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion. EMBO J 17(9):2494-503
3) Sacher M, et al.  (2001) TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell 7(2):433-42
4) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6
5) Shirahama-Noda K, et al.  (2013) TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy. J Cell Sci 126(Pt 21):4963-73
6) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94