GPT2/YKR067W Summary Help

Standard Name GPT2
Systematic Name YKR067W
Alias GAT1 1
Feature Type ORF, Verified
Description Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen (1, 2, 3, 4)
Name Description Glycerol-3-Phosphate acylTransferase
Chromosomal Location
ChrXI:567918 to 570149 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GPT2 GO evidence and references
  View Computational GO annotations for GPT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
137 total interaction(s) for 99 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • PCA: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 102
  • Phenotypic Suppression: 1
  • Positive Genetic: 17
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 743
Molecular Weight (Da) 83,644
Isoelectric Point (pI) 10.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:567918 to 570149 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2232 567918..570149 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001775
References cited on this page View Complete Literature Guide for GPT2
1) Zheng Z and Zou J  (2001) The initial step of the glycerolipid pathway: identification of glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae. J Biol Chem 276(45):41710-6
2) Athenstaedt K and Daum G  (1997) Biosynthesis of phosphatidic acid in lipid particles and endoplasmic reticulum of Saccharomyces cerevisiae. J Bacteriol 179(24):7611-6
3) Athenstaedt K, et al.  (1999) Redundant systems of phosphatidic acid biosynthesis via acylation of glycerol-3-phosphate or dihydroxyacetone phosphate in the yeast Saccharomyces cerevisiae. J Bacteriol 181(5):1458-63
4) Pagac M, et al.  (2012) Topology of the microsomal glycerol-3-phosphate acyltransferase Gpt2p/Gat1p of Saccharomyces cerevisiae. Mol Microbiol 86(5):1156-66