NAP1/YKR048C Summary Help

Standard Name NAP1 1
Systematic Name YKR048C
Feature Type ORF, Verified
Description Histone chaperone; involved in histone exchange by removing and replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; involved in the transport of H2A and H2B histones to the nucleus; required for the regulation of microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6, 7)
Name Description Nucleosome Assembly Protein 1
Chromosomal Location
ChrXI:526640 to 525387 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NAP1 GO evidence and references
  View Computational GO annotations for NAP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
451 total interaction(s) for 260 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 153
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 40
  • Biochemical Activity: 12
  • Co-crystal Structure: 1
  • PCA: 14
  • Reconstituted Complex: 14
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 127
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 3
  • Positive Genetic: 17
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 4
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 417
Molecular Weight (Da) 47,884
Isoelectric Point (pI) 4.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:526640 to 525387 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1254 526640..525387 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001756
References cited on this page View Complete Literature Guide for NAP1
1) Ishimi Y and Kikuchi A  (1991) Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. J Biol Chem 266(11):7025-9
2) Kellogg DR and Murray AW  (1995) NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast. J Cell Biol 130(3):675-85
3) Fujii-Nakata T, et al.  (1992) Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity. J Biol Chem 267(29):20980-6
4) Mosammaparast N, et al.  (2002) A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J 21(23):6527-38
5) Calvert ME, et al.  (2008) Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol 28(4):1313-25
6) Park YJ, et al.  (2005) Nucleosome assembly protein 1 exchanges histone H2A-H2B dimers and assists nucleosome sliding. J Biol Chem 280(3):1817-25
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76