KAE1/YKR038C Summary Help

Standard Name KAE1 1
Systematic Name YKR038C
Feature Type ORF, Verified
Description Highly conserved ATPase of HSP70/DnaK family; essential functional component of the EKC/KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Kinase-Associated Endopeptidase 1
Chromosomal Location
ChrXI:513159 to 511999 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All KAE1 GO evidence and references
  View Computational GO annotations for KAE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
reduction of function
unspecified
Large-scale survey
conditional
null
reduction of function
Resources
55 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Far Western: 3
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 10
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 386
Molecular Weight (Da) 42,748
Isoelectric Point (pI) 7.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:513159 to 511999 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1161 513159..511999 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001746
References cited on this page View Complete Literature Guide for KAE1
1) Lopreiato R, et al.  (2004) Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin. Biochem J 377(Pt 2):395-405
2) Downey M, et al.  (2006) A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell 124(6):1155-68
3) Kisseleva-Romanova E, et al.  (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J 25(15):3576-85
4) Oberto J, et al.  (2009) Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res 37(16):5343-52
5) Srinivasan M, et al.  (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
6) Daugeron MC, et al.  (2011) Gcn4 misregulation reveals a direct role for the evolutionary conserved EKC/KEOPS in the t6A modification of tRNAs. Nucleic Acids Res 39(14):6148-60
7) Hu Y, et al.  (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLoS Genet 9(1):e1003208
8) Wan LC, et al.  (2013) Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res 41(12):6332-46