SPC34/YKR037C Summary Help

Standard Name SPC34
Systematic Name YKR037C
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body (1, 2, 3, 4, 5)
Name Description Spindle Pole Component
Chromosomal Location
ChrXI:511797 to 510910 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPC34 GO evidence and references
  View Computational GO annotations for SPC34
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
129 total interaction(s) for 50 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • Co-purification: 31
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 37

Genetic Interactions
  • Dosage Growth Defect: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 295
Molecular Weight (Da) 34,077
Isoelectric Point (pI) 8.6
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:511797 to 510910 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..888 511797..510910 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001745
References cited on this page View Complete Literature Guide for SPC34
1) Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
2) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
3) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9