DAL80/YKR034W Summary Help

Standard Name DAL80
Systematic Name YKR034W
Alias UGA43 1
Feature Type ORF, Verified
Description Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication (2, 3, 4, 5 and see Summary Paragraph)
Name Description Degradation of Allantoin
Chromosomal Location
ChrXI:506898 to 507707 | ORF Map | GBrowse
Genetic position: 14 cM
Gene Ontology Annotations All DAL80 GO evidence and references
  View Computational GO annotations for DAL80
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 12 genes
Regulators 7 genes
Classical genetics
reduction of function
Large-scale survey
60 total interaction(s) for 57 unique genes/features.
Physical Interactions
  • Two-hybrid: 27

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 28
  • Positive Genetic: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 269
Molecular Weight (Da) 30,166
Isoelectric Point (pI) 8.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:506898 to 507707 | ORF Map | GBrowse
Genetic position: 14 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..810 506898..507707 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001742

DAL80 encodes a transcriptional repressor that is involved in negatively regulating genes that are subject to nitrogen catobolite repression (NCR) (6 and reviewed in 7). Dal80p, via a zinc finger binding domain, binds as a dimer to the URS-GATA found in the promoters of many genes involved in nitrogen utilization (6, 3). The URS-GATA consists of a pair of 5-GATAA-3 sequences separated by at least 15bp with a preferential tail to tail orientation (8). Dal80p is able to from heterodimeric complexes with Gzf3p, which may influence the DNA affinity of these proteins (3). Dal80p targets include genes involved in the import and catabolism of glutamine, glutamate, proline, urea, arginine, GABA, and allantoin, as well as genes involved in vacuolar protein degradation (6, 9, 10 and references therein).

Dal80p is one of four GATA family members which are involved in mediating nitrogen-responsive gene expression; Gln3p and Gat1p acting as activators in opposition to the Dal80p and Gzf3p repressors (reviewed in 7). Gat1p and Dal80p/Gzf3p are able to directly compete for GATA binding sites (11). Additionally, these gene products transcriptionally regulate each other and themselves in an interdependent fashion. The promoters of the GAT1, DAL80, and GZF3 genes all contain multiple GATA sequences (12, 13, 6): DAL80 expression is regulated by Gln3p, Gat1p,and Dal80p (13, 6); GAT1 expression is Gln3p and Dal80p regulated; GZF3 expression is moderately dependent on Gat1p and highly regulated by Dal80p (14). The expression levels themselves of GAT1 and DAL80 seem to regulate each other and are inversely correlated (15, 12).

Dal80p is similar to transcriptional regulators found in other fungi, birds, and mammals (16, 6). In other organisms, regulation of nitrogen metabolism may play a role in their pathogenicity (2).

Last updated: 2010-05-17 Contact SGD

References cited on this page View Complete Literature Guide for DAL80
1) Coornaert D, et al.  (1992) The UGA43 negative regulatory gene of Saccharomyces cerevisiae contains both a GATA-1 type zinc finger and a putative leucine zipper. Curr Genet 21(4-5):301-7
2) Marzluf GA  (1997) Genetic regulation of nitrogen metabolism in the fungi. Microbiol Mol Biol Rev 61(1):17-32
3) Svetlov VV and Cooper TG  (1998) The Saccharomyces cerevisiae GATA factors Dal80p and Deh1p can form homo- and heterodimeric complexes. J Bacteriol 180(21):5682-8
4) Magasanik B and Kaiser CA  (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Cunningham TS and Cooper TG  (1991) Expression of the DAL80 gene, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression. Mol Cell Biol 11(12):6205-15
7) Hofman-Bang J  (1999) Nitrogen catabolite repression in Saccharomyces cerevisiae. Mol Biotechnol 12(1):35-73
8) Cunningham TS and Cooper TG  (1993) The Saccharomyces cerevisiae DAL80 repressor protein binds to multiple copies of GATAA-containing sequences (URSGATA). J Bacteriol 175(18):5851-61
9) Chisholm G and Cooper TG  (1982) Isolation and characterization of mutants that produce the allantoin-degrading enzymes constitutively in Saccharomyces cerevisiae. Mol Cell Biol 2(9):1088-95
10) Coffman JA and Cooper TG  (1997) Nitrogen GATA factors participate in transcriptional regulation of vacuolar protease genes in Saccharomyces cerevisiae. J Bacteriol 179(17):5609-13
11) Georis I, et al.  (2009) The yeast GATA factor Gat1 occupies a central position in nitrogen catabolite repression-sensitive gene activation. Mol Cell Biol 29(13):3803-15
12) Cunningham TS, et al.  (2000) The level of DAL80 expression down-regulates GATA factor-mediated transcription in Saccharomyces cerevisiae. J Bacteriol 182(23):6584-91
13) Coffman JA, et al.  (1996) Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae. Mol Cell Biol 16(3):847-58
14) Coffman JA, et al.  (1997) Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J Bacteriol 179(11):3416-29
15) Cunningham TS, et al.  (2000) Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gat1p and Ure2p production in Saccharomyces cerevisiae. J Biol Chem 275(19):14408-14
16) Haas H, et al.  (1997) Overexpression of nreB, a new GATA factor-encoding gene of Penicillium chrysogenum, leads to repression of the nitrate assimilatory gene cluster. J Biol Chem 272(36):22576-82
17) Cunningham TS, et al.  (1994) The UGA4 UASNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae. J Bacteriol 176(15):4718-25
18) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87