SPO14/YKR031C Summary Help

Standard Name SPO14 1
Systematic Name YKR031C
Alias PLD1
Feature Type ORF, Verified
Description Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions (2, 3, 4, 5, 6)
Name Description SPOrulation 7
Chromosomal Location
ChrXI:506395 to 501344 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 18 cM
Gene Ontology Annotations All SPO14 GO evidence and references
  View Computational GO annotations for SPO14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
94 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-localization: 2
  • Co-purification: 1
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 24
  • Phenotypic Suppression: 8
  • Positive Genetic: 3
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,683
Molecular Weight (Da) 195,202
Isoelectric Point (pI) 7.58
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:506395 to 501344 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 18 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..5052 506395..501344 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001739
References cited on this page View Complete Literature Guide for SPO14
1) Townsend, M., et al.  (1985) Personal Communication, Mortimer Map Edition 9
2) Xie Z, et al.  (1998) Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. Proc Natl Acad Sci U S A 95(21):12346-51
3) Sreenivas A, et al.  (1998) A role for phospholipase D (Pld1p) in growth, secretion, and regulation of membrane lipid synthesis in yeast. J Biol Chem 273(27):16635-8
4) Rudge SA, et al.  (1998) Relocalization of phospholipase D activity mediates membrane formation during meiosis. J Cell Biol 140(1):81-90
5) Rudge SA, et al.  (2002) Differential regulation of Saccharomyces cerevisiae phospholipase D in sporulation and Sec14-independent secretion. Genetics 160(4):1353-61
6) Garcia-Lopez MC, et al.  (2011) The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation. Genetics 189(4):1235-48
7) Esposito MS, et al.  (1970) Conditional mutants of meiosis in yeast. J Bacteriol 104(1):202-10