TOF2/YKR010C Summary Help

Standard Name TOF2 1
Systematic Name YKR010C
Feature Type ORF, Verified
Description Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description TOpoisomerase I-interacting Factor 1
Chromosomal Location
ChrXI:461239 to 458924 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TOF2 GO evidence and references
  View Computational GO annotations for TOF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
148 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 75
  • Positive Genetic: 10
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 6
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 771
Molecular Weight (Da) 86,365
Isoelectric Point (pI) 8.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:461239 to 458924 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2316 461239..458924 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001718
References cited on this page View Complete Literature Guide for TOF2
1) Park H and Sternglanz R  (1999) Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae. Yeast 15(1):35-41
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Huang J, et al.  (2006) Inhibition of homologous recombination by a cohesin-associated clamp complex recruited to the rDNA recombination enhancer. Genes Dev 20(20):2887-901
4) Geil C, et al.  (2008) A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis. Curr Biol 18(13):1001-5
5) Waples WG, et al.  (2009) Putting the Brake on FEAR: Tof2 Promotes the Biphasic Release of Cdc14 Phosphatase during Mitotic Exit. Mol Biol Cell 20(1):245-55
6) Johzuka K and Horiuchi T  (2009) The cis element and factors required for condensin recruitment to chromosomes. Mol Cell 34(1):26-35