JEN1/YKL217W Summary Help

Standard Name JEN1 1
Systematic Name YKL217W
Feature Type ORF, Verified
Description Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose (2, 3, 4, 5, 6, 7, 8, 9)
Chromosomal Location
ChrXI:22234 to 24084 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All JEN1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
36 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 2
  • PCA: 1

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 22
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 616
Molecular Weight (Da) 69,376
Isoelectric Point (pI) 5.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:22234 to 24084 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1851 22234..24084 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000001700
References cited on this page View Complete Literature Guide for JEN1
1) Davis, E.S.  (1994) Molecular Analysis of Cyclophilin Function in the yeast Saccharomyces cerevisiae. Ph.D. Thesis, University of Maryland
2) Haurie V, et al.  (2001) The transcriptional activator Cat8p provides a major contribution to the reprogramming of carbon metabolism during the diauxic shift in Saccharomyces cerevisiae. J Biol Chem 276(1):76-85
3) Casal M, et al.  (1999) The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. J Bacteriol 181(8):2620-3
4) Akita O, et al.  (2000) Transport of pyruvate in Saccharomyces cerevisiae and cloning of the gene encoded pyruvate permease. Biosci Biotechnol Biochem 64(5):980-4
5) Paiva S, et al.  (2002) Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae. Biochem J 363(Pt 3):737-44
6) Chambers P, et al.  (2004) Saccharomyces cerevisiae JEN1 promoter activity is inversely related to concentration of repressing sugar. Appl Environ Microbiol 70(1):8-17
7) Andrade RP, et al.  (2005) Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae. Biochem Biophys Res Commun 332(1):254-62
8) Soares-Silva I, et al.  (2007) The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H(+) symporter subfamily defines the function of the substrate translocation pathway. Mol Membr Biol 24(5):464-74
9) McDermott JR, et al.  (2010) Jen1p: a high affinity selenite transporter in yeast. Mol Biol Cell 21(22):3934-41