Standard Name	DOA1 1
Systematic Name	YKL213C
Alias	UFD3 2 , ZZZ4 3
Feature Type	ORF, Verified
Description	WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress (2, 4, 5, 6)
Name Description	Degradation Of Alpha 1

Chromosomal Location	ChrXI:34107 to 31960 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for DOA1
Molecular Function
Manually curated	ubiquitin binding (IDA, IMP)
Biological Process
Manually curated	double-strand break repair via nonhomologous end joining (IMP) ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	UV resistance: decreased tellurium atom accumulation: increased prion formation: decreased resistance to isoflurane: increased resistance to methyl methanesulfonate: decreased resistance to 4-aminofolic acid: decreased
Large-scale survey
null	UV resistance: decreased acid pH resistance: decreased auxotrophy glycogen accumulation: decreased glutathione excretion: increased competitive fitness: decreased heat sensitivity: increased ionic stress resistance: decreased lipid particle morphology: abnormal metal resistance: decreased resistance to fluconazole: increased resistance to fenpropimorph: increased resistance to caffeine: increased resistance to camptothecin: increased resistance to quinine: increased resistance to cycloheximide: decreased resistance to mycophenolic acid: increased resistance to methyl methanesulfonate: decreased resistance to propan-1-ol: decreased resistance to arsenite(3-): decreased resistance to tunicamycin: decreased resistance to boric acid: decreased resistance to BPS: decreased resistance to elesclomol-Cu: decreased resistance to hydroxyurea: decreased resistance to L-1,4-dithiothreitol: decreased resistance to caffeine: decreased resistance to 1,4-dithiothreitol: decreased sporulation: absent sporulation: decreased sporulation efficiency: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	767 total interaction(s) for 437 unique genes/features.
Physical Interactions	Affinity Capture-MS: 15 Affinity Capture-RNA: 1 Affinity Capture-Western: 19 Co-purification: 4 Protein-peptide: 1 Reconstituted Complex: 7 Two-hybrid: 11
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 6 Negative Genetic: 520 Phenotypic Enhancement: 13 Phenotypic Suppression: 15 Positive Genetic: 86 Synthetic Growth Defect: 49 Synthetic Lethality: 9 Synthetic Rescue: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:34107 to 31960 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2148 34107..31960 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001696