DOA1/YKL213C Summary Help

Standard Name DOA1 1
Systematic Name YKL213C
Alias UFD3 2 , ZZZ4 3
Feature Type ORF, Verified
Description WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; facilitates N-terminus-dependent proteolysis of centromeric histone H3 (Cse4p) for faithful chromosome segregation; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress (2, 4, 5, 6, 7)
Name Description Degradation Of Alpha 1
Chromosomal Location
ChrXI:34107 to 31960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DOA1 GO evidence and references
  View Computational GO annotations for DOA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 1 genes
Classical genetics
Large-scale survey
779 total interaction(s) for 441 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Co-crystal Structure: 1
  • Co-purification: 4
  • Protein-peptide: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 520
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 16
  • Positive Genetic: 86
  • Synthetic Growth Defect: 51
  • Synthetic Lethality: 9
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 715
Molecular Weight (Da) 79,505
Isoelectric Point (pI) 4.71
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:34107 to 31960 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2148 34107..31960 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001696
References cited on this page View Complete Literature Guide for DOA1
1) Hochstrasser M and Varshavsky A  (1990) In vivo degradation of a transcriptional regulator: the yeast alpha 2 repressor. Cell 61(4):697-708
2) Johnson ES, et al.  (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270(29):17442-56
3) Keil RL, et al.  (1996) Molecular genetic analysis of volatile-anesthetic action. Mol Cell Biol 16(7):3446-53
4) Ghislain M, et al.  (1996) Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae. EMBO J 15(18):4884-99
5) Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Au WC, et al.  (2013) A novel role of the N terminus of budding yeast histone h3 variant cse4 in ubiquitin-mediated proteolysis. Genetics 194(2):513-8