LOS1/YKL205W Summary Help

Standard Name LOS1 1
Systematic Name YKL205W
Feature Type ORF, Verified
Description Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm (2, 3, 4, 5)
Name Description Loss Of Suppression 1
Chromosomal Location
ChrXI:50051 to 53353 | ORF Map | GBrowse
Gbrowse
Genetic position: -149.7 cM
Gene Ontology Annotations All LOS1 GO evidence and references
  View Computational GO annotations for LOS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
114 total interaction(s) for 82 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 4
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 41
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 6
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,100
Molecular Weight (Da) 126,818
Isoelectric Point (pI) 4.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:50051 to 53353 | ORF Map | GBrowse
SGD ORF map
Genetic position: -149.7 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3303 50051..53353 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001688
References cited on this page View Complete Literature Guide for LOS1
1) Hopper AK, et al.  (1980) Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs. Cell 19(3):741-51
2) Hurt DJ, et al.  (1987) Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol Cell Biol 7(3):1208-16
3) Hellmuth K, et al.  (1998) Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol 18(11):6374-86
4) Shen WC, et al.  (1993) The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix. J Biol Chem 268(26):19436-44
5) Murthi A, et al.  (2010) Regulation of tRNA Bidirectional Nuclear-Cytoplasmic Trafficking in Saccharomyces cerevisiae. Mol Biol Cell 21(4):639-49