EAP1/YKL204W Summary Help

Standard Name EAP1 1, 2
Systematic Name YKL204W
Feature Type ORF, Verified
Description eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade (1, 2, 3, 4)
Name Description EIF4E-Associated Protein 1, 2
Chromosomal Location
ChrXI:53704 to 55602 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EAP1 GO evidence and references
  View Computational GO annotations for EAP1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
78 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Co-fractionation: 1
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 13
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 13
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 632
Molecular Weight (Da) 69,762
Isoelectric Point (pI) 10.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:53704 to 55602 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1899 53704..55602 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001687
References cited on this page View Complete Literature Guide for EAP1
1) Cosentino GP, et al.  (2000) Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae. Mol Cell Biol 20(13):4604-13
2) Chial HJ, et al.  (2000) Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability. Curr Biol 10(23):1519-22
3) Blewett NH and Goldstrohm AC  (2012) A eukaryotic translation initiation factor 4E-binding protein promotes mRNA decapping and is required for PUF repression. Mol Cell Biol 32(20):4181-94
4) Rendl LM, et al.  (2012) The eIF4E-Binding Protein Eap1p Functions in Vts1p-Mediated Transcript Decay. PLoS One 7(10):e47121