CNB1/YKL190W Summary Help

Standard Name CNB1 1
Systematic Name YKL190W
Alias CRV1 , YCN2
Feature Type ORF, Verified
Description Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description CalciNeurin subunit B
Chromosomal Location
ChrXI:82947 to 83550 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CNB1 GO evidence and references
  View Computational GO annotations for CNB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
319 total interaction(s) for 221 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 1
  • PCA: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 15
  • Negative Genetic: 175
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 5
  • Positive Genetic: 7
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 51
  • Synthetic Rescue: 14

Resources
Expression Summary
histogram
Resources
Length (a.a.) 175
Molecular Weight (Da) 19,639
Isoelectric Point (pI) 4.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:82947 to 83550 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..52 82947..82998 2011-02-03 1996-07-31
Intron 53..128 82999..83074 2011-02-03 1996-07-31
CDS 129..604 83075..83550 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001673
References cited on this page View Complete Literature Guide for CNB1
1) Cyert MS and Thorner J  (1992) Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone. Mol Cell Biol 12(8):3460-9
2) Cyert MS  (2003) Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress. Biochem Biophys Res Commun 311(4):1143-50
3) Rusnak F and Mertz P  (2000) Calcineurin: form and function. Physiol Rev 80(4):1483-521
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Connolly S and Kingsbury T  (2012) Regulatory subunit myristoylation antagonizes calcineurin phosphatase activation in yeast. J Biol Chem 287(47):39361-8
6) O'Donnell AF, et al.  (2013) A Calcineurin-dependent Switch Controls the Trafficking Function of a-Arrestin Aly1/Art6. J Biol Chem 288(33):24063-80