SNU114/YKL173W Summary Help

Standard Name SNU114 1
Systematic Name YKL173W
Alias GIN10
Feature Type ORF, Verified
Description GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 (1, 2, 3, 4)
Name Description Small NUclear ribonucleoprotein associated 1
Chromosomal Location
ChrXI:122517 to 125543 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SNU114 GO evidence and references
  View Computational GO annotations for SNU114
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
conditional
null
reduction of function
Resources
254 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 167
  • Affinity Capture-Western: 9
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 3
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 42
  • Positive Genetic: 5
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,008
Molecular Weight (Da) 114,040
Isoelectric Point (pI) 5.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:122517 to 125543 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3027 122517..125543 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001656
References cited on this page View Complete Literature Guide for SNU114
1) Fabrizio P, et al.  (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106
2) Dix I, et al.  (1998) Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4(12):1675-86
3) Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
4) Brenner TJ and Guthrie C  (2005) Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics 170(3):1063-80