SNU114/YKL173W Summary

SNU114 BASIC INFORMATION

Standard Name	SNU114 1
Systematic Name	YKL173W
Alias	GIN10
Feature Type	ORF, Verified
Description	GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 (1, 2, 3, 4)
Name Description	Small NUclear ribonucleoprotein associated 1

GO Annotations	All SNU114 GO evidence and references
	View Computational GO annotations for SNU114
Molecular Function
Manually curated	GTP binding (ISS) GTPase activity (IMP, ISS) U5 snRNA binding (IDA)
Biological Process
Manually curated	assembly of spliceosomal tri-snRNP (IMP) generation of catalytic spliceosome for first transesterification step (IGI) nuclear mRNA splicing, via spliceosome (IMP) spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) (IMP)
Cellular Component
Manually curated	U4/U6 x U5 tri-snRNP complex (IDA) U5 snRNP (IDA)

Mutant Phenotype	All SNU114 Phenotype details and references
Large-scale survey
null	inviable

Interactions	SNU114 All interactions details and references
	188 total interaction(s) for 82 unique genes/features.
Physical Interactions	Affinity Capture-MS: 148 Affinity Capture-Western: 9 Co-fractionation: 2 Co-localization: 1 Co-purification: 3 PCA: 3 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Synthetic Lethality: 12

Sequence Information

ChrXI:122522 to 125548 | |

Last Update

Coordinates: 2004-02-13 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3027	122522..125548	2004-02-13	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001656

SNU114 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SNU114

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SNU114]

1)	Fabrizio P, et al. (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106
2)	Dix I, et al. (1998) Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4(12):1675-86
3)	Stevens SW and Abelson J (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
4)	Brenner TJ and Guthrie C (2005) Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics 170(3):1063-80

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