EBP2/YKL172W Summary Help

Standard Name EBP2 1
Systematic Name YKL172W
Feature Type ORF, Verified
Description Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering (1, 2, 3, 4)
Name Description EBNA1-binding protein (homolog) 1
Chromosomal Location
ChrXI:125759 to 127042 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EBP2 GO evidence and references
  View Computational GO annotations for EBP2
Molecular Function
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
null
overexpression
unspecified
Resources
180 total interaction(s) for 111 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 88
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 14
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 15
  • Reconstituted Complex: 1
  • Two-hybrid: 39

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 1
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 427
Molecular Weight (Da) 49,734
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:125759 to 127042 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1284 125759..127042 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001655
References cited on this page View Complete Literature Guide for EBP2
1) Huber MD, et al.  (2000) The budding yeast homolog of the human EBNA1-binding protein 2 (Ebp2p) is an essential nucleolar protein required for pre-rRNA processing. J Biol Chem 275(37):28764-73
2) Tsujii R, et al.  (2000) Ebp2p, yeast homologue of a human protein that interacts with Epstein-Barr virus nuclear antigen 1, is required for pre-rRNA processing and ribosomal subunit assembly. Genes Cells 5(7):543-53
3) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4) Horigome C, et al.  (2011) Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres.LID - 10.1038/emboj.2011.267 [doi] EMBO J ()