NNK1/YKL171W Summary Help

Standard Name NNK1 1
Systematic Name YKL171W
Feature Type ORF, Verified
Description Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm (1, 2, 3, 4, 5)
Name Description Nitrogen Network Kinase 1
Chromosomal Location
ChrXI:127475 to 130261 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NNK1 GO evidence and references
  View Computational GO annotations for NNK1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
High-throughput
Regulators 4 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
132 total interaction(s) for 98 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Biochemical Activity: 43
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 4
  • Negative Genetic: 46
  • Positive Genetic: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 928
Molecular Weight (Da) 103,955
Isoelectric Point (pI) 9.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:127475 to 130261 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2787 127475..130261 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001654
References cited on this page View Complete Literature Guide for NNK1
1) Breitkreutz A, et al.  (2010) A global protein kinase and phosphatase interaction network in yeast. Science 328(5981):1043-6
2) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
3) Cagney G, et al.  (2001) Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome. Physiol Genomics 7(1):27-34
4) Kumar A, et al.  (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19
5) Miranda-Saavedra D and Barton GJ  (2007) Classification and functional annotation of eukaryotic protein kinases. Proteins 68(4):893-914