APE2/YKL157W Summary Help

Standard Name APE2
Systematic Name YKL157W
Alias LAP1 , YKL158W
Feature Type ORF, Verified
Description Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication (1, 2, 3, 4 and see Summary Paragraph)
Name Description AminoPEptidase
Chromosomal Location
ChrXI:154991 to 158232 | ORF Map | GBrowse
Gbrowse
Genetic position: -124.8 cM
Gene Ontology Annotations All APE2 GO evidence and references
  View Computational GO annotations for APE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Large-scale survey
null
overexpression
Resources
89 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 57
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 952
Molecular Weight (Da) 107,755
Isoelectric Point (pI) 8.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:154991 to 158232 | ORF Map | GBrowse
This feature contains embedded feature(s): YKL158W
SGD ORF map
Genetic position: -124.8 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..281 154991..155271 2011-02-03 2000-07-14
Intron 282..664 155272..155654 2011-02-03 2000-07-14
CDS 665..3242 155655..158232 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001640
SUMMARY PARAGRAPH for APE2

APE2 encodes one of four leucine aminopeptidases in yeast (5). It has sequence similarity to members of the zinc-dependent metallopeptidase gene family (3). Ape2p seems to be involved in peptide metabolism, in the cellular supply of leucine from externally offered leucine-containing dipeptide substrates (2, 5). Ape2p has also been referred to as Lap1p, and aminopeptidase yscII (3). Ape2p is distributed in the periplasmic space and cytoplasm (3).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for APE2
1) Davis CA, et al.  (2000) Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast. Nucleic Acids Res 28(8):1700-6
2) Hirsch HH, et al.  (1988) Aminopeptidase yscII of yeast. Isolation of mutants and their biochemical and genetic analysis. Eur J Biochem 173(3):589-98
3) Garcia-Alvarez N, et al.  (1991) Molecular cloning of soluble aminopeptidases from Saccharomyces cerevisiae. Sequence analysis of aminopeptidase yscII, a putative zinc-metallopeptidase. Eur J Biochem 202(3):993-1002
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Trumbly RJ and Bradley G  (1983) Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae. J Bacteriol 156(1):36-48