APL2/YKL135C Summary Help

Standard Name APL2 1
Systematic Name YKL135C
Feature Type ORF, Verified
Description Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description clathrin Adaptor Protein complex Large chain 1
Chromosomal Location
ChrXI:188640 to 186460 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All APL2 GO evidence and references
  View Computational GO annotations for APL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
145 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 18
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 42
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 726
Molecular Weight (Da) 81,868
Isoelectric Point (pI) 4.95
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:188640 to 186460 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2181 188640..186460 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001618
References cited on this page View Complete Literature Guide for APL2
1) Rad MR, et al.  (1995) Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions. J Cell Sci 108 ( Pt 4)():1605-15
2) Kirchhausen T  (2000) Three ways to make a vesicle. Nat Rev Mol Cell Biol 1(3):187-98
3) Yeung BG, et al.  (1999) Adaptor complex-independent clathrin function in yeast. Mol Biol Cell 10(11):3643-59
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76