YPK1/YKL126W Summary Help

Standard Name YPK1
Systematic Name YKL126W
Alias SLI2
Feature Type ORF, Verified
Description Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Yeast Protein Kinase
Chromosomal Location
ChrXI:205707 to 207749 | ORF Map | GBrowse
Genetic position: -97.7 cM
Gene Ontology Annotations All YPK1 GO evidence and references
  View Computational GO annotations for YPK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
dominant negative
Large-scale survey
115 total interaction(s) for 85 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 9
  • Co-fractionation: 2
  • PCA: 1
  • Reconstituted Complex: 4

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 23
  • Negative Genetic: 3
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 9
  • Synthetic Rescue: 9

Expression Summary
Length (a.a.) 680
Molecular Weight (Da) 76,479
Isoelectric Point (pI) 6.47
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:205707 to 207749 | ORF Map | GBrowse
Genetic position: -97.7 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2043 205707..207749 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001609
References cited on this page View Complete Literature Guide for YPK1
1) Sun Y, et al.  (2000) Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Mol Cell Biol 20(12):4411-9
2) Casamayor A, et al.  (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
3) deHart AK, et al.  (2002) The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast. J Cell Biol 156(2):241-8
4) Schmelzle T, et al.  (2002) Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol 22(5):1329-39
5) Roelants FM, et al.  (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Roelants FM, et al.  (2010) A protein kinase network regulates the function of aminophospholipid flippases. Proc Natl Acad Sci U S A 107(1):34-9
8) Roelants FM, et al.  (2011) Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 108(48):19222-7