SSH4/YKL124W Summary Help

Standard Name SSH4 1
Systematic Name YKL124W
Alias MLF4 2
Feature Type ORF, Verified
Description Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases (1, 3)
Name Description Suppressor of SHr3 deletion 1
Chromosomal Location
ChrXI:210593 to 212332 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SSH4 GO evidence and references
  View Computational GO annotations for SSH4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
null
overexpression
Resources
21 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 9
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 579
Molecular Weight (Da) 65,060
Isoelectric Point (pI) 4.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:210593 to 212332 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1740 210593..212332 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001607
References cited on this page View Complete Literature Guide for SSH4
1) Kota J, et al.  (2007) Ssh4, Rcr2 and Rcr1 Affect Plasma Membrane Transporter Activity in Saccharomyces cerevisiae. Genetics 175(4):1681-94
2) Fujimura H  (1998) Molecular cloning of Saccharomyces cerevisiae MLF4/SSH4 gene which confers the immunosuppressant leflunomide resistance. Biochem Biophys Res Commun 246(2):378-81
3) Leon S, et al.  (2008) Ear1p and ssh4p are new adaptors of the ubiquitin ligase rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Mol Biol Cell 19(6):2379-88