VPH2/YKL119C Summary Help

Standard Name VPH2 1
Systematic Name YKL119C
Alias CLS10 , VMA12
Feature Type ORF, Verified
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner (1, 2, 3, 4, 5)
Name Description Vacuolar pH 1
Chromosomal Location
ChrXI:219217 to 218570 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPH2 GO evidence and references
  View Computational GO annotations for VPH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
284 total interaction(s) for 219 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • PCA: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 197
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 51
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 215
Molecular Weight (Da) 25,286
Isoelectric Point (pI) 9.78
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:219217 to 218570 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..648 219217..218570 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001602
References cited on this page View Complete Literature Guide for VPH2
1) Preston RA, et al.  (1992) Genes required for vacuolar acidity in Saccharomyces cerevisiae. Genetics 131(3):551-8
2) Jackson DD and Stevens TH  (1997) VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly. J Biol Chem 272(41):25928-34
3) Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4
4) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47
5) Ruckenstuhl C, et al.  (2014) Lifespan extension by methionine restriction requires autophagy-dependent vacuolar acidification. PLoS Genet 10(5):e1004347