Standard Name	HAP4 1
Systematic Name	YKL109W
Feature Type	ORF, Verified
Description	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex (1)
Name Description	Heme Activator Protein 1

Chromosomal Location	ChrXI:232227 to 233891 | ORF Map | GBrowse

	View Computational GO annotations for HAP4
Molecular Function
Manually curated	RNA polymerase II activating transcription factor binding (IDA, IPI) contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA, IMP) RNA polymerase II transcription factor binding transcription factor activity (IC)
Biological Process
Manually curated	carbon catabolite activation of transcription from RNA polymerase II promoter (IMP) regulation of cellular respiration (IMP)
Cellular Component
Manually curated	CCAAT-binding factor complex (IDA)

Binding motifs	Predicted HAP4 Binding Site Locations
	GNCCAATCA 2 [Search intergenic regions for GNCCAATCA] YCNNCCAATNANM 3 [Search intergenic regions for YCNNCCAATNANM]
Regulatory modules	predicted: stressResponse (625, 619, 481)
Resources	ScerTF | YEASTRACT | YeTFaSCo

Classical genetics
null	replicative lifespan: decreased resistance to methyl methanesulfonate: decreased respiratory metabolism: decreased utilization of carbon source: decreased viable
overexpression	survival rate in stationary phase: increased
reduction of function	utilization of carbon source: decreased
Large-scale survey
null	UV resistance: decreased anaerobic growth: decreased bud morphology: abnormal cell size: decreased competitive fitness: decreased dessication resistance: decreased filamentous growth: decreased haploinsufficient invasive growth: absent Fus-Mid-GFP distribution: abnormal resistance to propolis extract: decreased resistance to ethanol: decreased respiratory growth: decreased rate respiratory growth: absent sporulation efficiency: decreased utilization of carbon source: decreased vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	128 total interaction(s) for 116 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 2 Affinity Capture-Western: 7 Reconstituted Complex: 8 Two-hybrid: 7
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 77 Phenotypic Enhancement: 2 Phenotypic Suppression: 6 Positive Genetic: 11 Synthetic Growth Defect: 1 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:232227 to 233891 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1665 232227..233891 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001592