APE1/YKL103C Summary Help

Standard Name APE1 1
Systematic Name YKL103C
Alias YSC1 , API , LAP4 1 , 2
Feature Type ORF, Verified
Description Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress (1, 2, 3, 4)
Name Description AminoPeptidase 1
Chromosomal Location
ChrXI:247682 to 246138 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -138 cM
Gene Ontology Annotations All APE1 GO evidence and references
  View Computational GO annotations for APE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 18 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
75 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Co-fractionation: 1
  • Co-localization: 3
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 28

Genetic Interactions
  • Negative Genetic: 8
  • Phenotypic Enhancement: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 514
Molecular Weight (Da) 57,092
Isoelectric Point (pI) 5.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:247682 to 246138 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -138 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1545 247682..246138 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001586
References cited on this page View Complete Literature Guide for APE1
1) Cueva R, et al.  (1989) Yeast vacuolar aminopeptidase yscI. Isolation and regulation of the APE1 (LAP4) structural gene. FEBS Lett 259(1):125-9
2) Schu P  (2008) Aminopeptidase I enzymatic activity. Methods Enzymol 451:67-78
3) Suzuki K, et al.  (2002) Studies of cargo delivery to the vacuole mediated by autophagosomes in Saccharomyces cerevisiae. Dev Cell 3(6):815-24
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76