YJU2/YKL095W Summary Help

Standard Name YJU2 1
Systematic Name YKL095W
Alias CWC16 2
Feature Type ORF, Verified
Description Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing (3, 4, 5)
Chromosomal Location
ChrXI:262277 to 263113 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YJU2 GO evidence and references
  View Computational GO annotations for YJU2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
repressible
Large-scale survey
conditional
null
reduction of function
repressible
Resources
60 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 40
  • Affinity Capture-Western: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 278
Molecular Weight (Da) 32,312
Isoelectric Point (pI) 10.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:262277 to 263113 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..837 262277..263113 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001578
References cited on this page View Complete Literature Guide for YJU2
1) Forrova H, et al.  (1992) Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae. Yeast 8(5):419-22
2) Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
5) Liu YC, et al.  (2007) A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol Cell Biol 27(15):5403-13