Standard Name	YJU2 1
Systematic Name	YKL095W
Alias	CWC16 2
Feature Type	ORF, Verified
Description	Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ("nineteen complex") and acts after Prp2p to promote the first catalytic reaction of splicing (3, 4, 5)

Chromosomal Location	ChrXI:262277 to 263113 | ORF Map | GBrowse

	View Computational GO annotations for YJU2
Molecular Function
Manually curated	contributes_to first spliceosomal transesterification activity (IDA)
Biological Process
Manually curated	generation of catalytic spliceosome for first transesterification step (IDA)
Cellular Component
Manually curated	U2-type catalytic step 1 spliceosome (IDA)
High-throughput	nucleus (IDA)

Classical genetics
null	inviable
repressible	unspliced pre-RNA accumulation: increased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
reduction of function	competitive fitness: increased resistance to methyl methanesulfonate: decreased
repressible	cell cycle progression in G1 phase: abnormal
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	60 total interaction(s) for 42 unique genes/features.
Physical Interactions	Affinity Capture-MS: 40 Affinity Capture-Western: 7 Reconstituted Complex: 1 Two-hybrid: 5
Genetic Interactions	Negative Genetic: 3 Phenotypic Enhancement: 1 Positive Genetic: 2 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:262277 to 263113 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..837 262277..263113 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001578