BUD2/YKL092C Summary Help

Standard Name BUD2 1
Systematic Name YKL092C
Alias CLA2 , ERC25
Feature Type ORF, Verified
Description GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types (1, 2, 3 and see Summary Paragraph)
Name Description BUD site selection
Chromosomal Location
ChrXI:269459 to 266145 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BUD2 GO evidence and references
  View Computational GO annotations for BUD2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
reduction of function
Large-scale survey
32 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4

Expression Summary
Length (a.a.) 1,104
Molecular Weight (Da) 126,662
Isoelectric Point (pI) 9.33
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:269459 to 266145 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3315 269459..266145 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001575

BUD2 encodes a GTPase-activating protein (GAP) for the small GTPase Rsr1p (4, 2). Along with the GTP/GDP exchange factor Bud5p, Bud2p regulates the activity of Rsr1p and thereby helps control bud site selection in growing cells (4). Deletion or over-expression of BUD2 causes yeast cells to bud randomly in all cell types, instead of in the usual axial pattern in haploids and bipolar pattern in a/alpha diploids (1, 2). When budding is conceptually divided into three steps, choice of a growth site, organization of the growth site, and actual growth and morphogenesis, Rsr1p and its negative regulator Bud2p and positive regulator Bud5p are the key players in the initial step of the process (5).

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for BUD2
1) Chant J and Herskowitz I  (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65(7):1203-12
2) Park HO, et al.  (1993) BUD2 encodes a GTPase-activating protein for Bud1/Rsr1 necessary for proper bud-site selection in yeast. Nature 365(6443):269-74
3) Nelson SA, et al.  (2012) A Novel Role for the GTPase-Activating Protein Bud2 in the Spindle Position Checkpoint. PLoS One 7(4):e36127
4) Bender A  (1993) Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast. Proc Natl Acad Sci U S A 90(21):9926-9
5) Cabib E, et al.  (1998) Role of small G proteins in yeast cell polarization and wall biosynthesis. Annu Rev Biochem 67:307-33