Standard Name	MIF2 1, 2 (see Nomenclature conflict Note)
Systematic Name	YKL089W
Feature Type	ORF, Verified
Description	Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 (1, 3, 4, 5)
Name Description	MItotic Fidelity of chromosome transmission 2

Chromosomal Location	ChrXI:273394 to 275043 | ORF Map | GBrowse
	Genetic position: -43 cM

	View Computational GO annotations for MIF2
Molecular Function
Manually curated	centromeric DNA binding (IDA)
Biological Process
Manually curated	chromosome segregation (IGI, IMP) mitotic spindle organization in nucleus (IMP)
Cellular Component
Manually curated	condensed nuclear chromosome inner kinetochore (IDA) condensed nuclear chromosome, centromeric region (IDA)

Classical genetics
conditional	cell cycle progression: arrested cell cycle progression in metaphase: arrested chromosome segregation: abnormal chromosome/plasmid maintenance: abnormal heat sensitivity: increased Mcd1p accumulation: abnormal Ctf19p distribution: abnormal Mtw1p distribution: abnormal resistance to methyl benzimidazol-2-yl-carbamate: decreased spindle morphology: abnormal viability: decreased
null	cell cycle progression: arrested inviable
overexpression	chromosome/plasmid maintenance: abnormal colony sectoring: increased
unspecified	inviable
Large-scale survey
conditional	colony sectoring: increased resistance to benomyl: decreased
null	inviable
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	85 total interaction(s) for 59 unique genes/features.
Physical Interactions	Affinity Capture-MS: 21 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Biochemical Activity: 5 Co-crystal Structure: 2 Co-localization: 1 Reconstituted Complex: 3 Two-hybrid: 14
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 1 Phenotypic Enhancement: 4 Positive Genetic: 1 Synthetic Growth Defect: 23 Synthetic Lethality: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:273394 to 275043 | ORF Map | GBrowse
	Genetic position: -43 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1650 273394..275043 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001572