Standard Name	MUD2 1
Systematic Name	YKL074C
Feature Type	ORF, Verified
Description	Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 (1, 2)
Name Description	Mutant U1 Die 3

Chromosomal Location	ChrXI:296193 to 294610 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for MUD2
Molecular Function
Manually curated	pre-mRNA branch point binding (IDA)
Biological Process
Manually curated	mRNA branch site recognition (IGI, IMP)
Cellular Component
Manually curated	commitment complex (IDA, IMP)

Classical genetics
null	unspliced pre-mRNA accumulation: increased unspliced pre-mRNA with branchpoint mutations localization: abnormal viable
Large-scale survey
null	glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased heat sensitivity: increased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to cycloheximide: increased resistance to methyl methanesulfonate: increased resistance to mycophenolic acid: increased resistance to bleomycin: decreased resistance to camptothecin: decreased resistance to caffeine: decreased resistance to sodium selenide: decreased resistance to dimethyl sulfoxide: decreased resistance to hydroxyurea: decreased sporulation efficiency: decreased stress resistance: increased toxin resistance: decreased vacuolar morphology: abnormal viable
overexpression	filamentous growth: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	231 total interaction(s) for 156 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-Western: 3 Co-fractionation: 1 Reconstituted Complex: 3 Two-hybrid: 25
Genetic Interactions	Negative Genetic: 118 Phenotypic Enhancement: 3 Phenotypic Suppression: 1 Positive Genetic: 22 Synthetic Growth Defect: 5 Synthetic Lethality: 10 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:296193 to 294610 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1584 296193..294610 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001557