MUD2/YKL074C Summary

MUD2 BASIC INFORMATION

Standard Name	MUD2 1
Systematic Name	YKL074C
Feature Type	ORF, Verified
Description	Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 (1, 2)
Name Description	Mutant U1 Die 3

GO Annotations	All MUD2 GO evidence and references
	View Computational GO annotations for MUD2
Molecular Function
Manually curated	pre-mRNA branch point binding (IDA)
Biological Process
Manually curated	nuclear mRNA branch site recognition (IGI, IMP)
Cellular Component
Manually curated	commitment complex (IDA, IMP)

Mutant Phenotype	All MUD2 Phenotype details and references
Classical genetics
null	unspliced pre-mRNA accumulation: increased unspliced pre-mRNA with branchpoint mutations localization: abnormal viable
Large-scale survey
null	glycogen accumulation: increased competitive fitness: decreased resistance to hydroxyurea: decreased viable
overexpression	filamentous growth: increased

Interactions	MUD2 All interactions details and references
	132 total interaction(s) for 89 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-Western: 3 Co-fractionation: 1 Reconstituted Complex: 3 Two-hybrid: 25
Genetic Interactions	Phenotypic Enhancement: 67 Phenotypic Suppression: 19 Synthetic Growth Defect: 4 Synthetic Lethality: 5 Synthetic Rescue: 2

Sequence Information

ChrXI:295837 to 294254 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-12-15 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1584	295837..294254	2005-12-15	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001557

MUD2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for MUD2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MUD2]

1)	Abovich N, et al. (1994) The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev 8(7):843-54
2)	Rutz B and Seraphin B (1999) Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly. RNA 5(6):819-31
3)	Liao XC, et al. (1993) An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing. Genes Dev 7(3):419-28

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