NUP100/YKL068W Summary Help

NUP100 BASIC INFORMATION

Standard Name NUP100
Systematic Name YKL068W
Alias NSP100
Feature Type ORF, Verified
Description Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup116p (1, 2 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP100 GO evidence and references
    View Computational GO annotations for NUP100
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP100 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions NUP100 All interactions details and references
120 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 51
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 4
  • Reconstituted Complex: 7
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 6
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1
Sequence Information
ChrXI:309843 to 312722 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2005-12-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2880 309843..312722 2005-12-15 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001551

NUP100 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP100

SUMMARY PARAGRAPH for NUP100

NUP100 encodes a nuclear pore protein (3, 4). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 4, 5, 6, 7). The structure of the vertebrate NPC has been studied extensively; recent reviews include 8, 9, 10, and 11. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (12, 13). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 4, 5, 14, 15, 16). NUP100 is not essential, but the nup100 deletion is synthetically lethal with mutations in genes encoding other nucleoporins and other nucleocytoplasmic transport factors (4, 17). Nup100p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (3, 4). Nup100p and Nup116p are similar over their entire lengths, and may have arisen by gene duplication (3, 18, 4). A related nucleoporin, Nup98, has been identified in vertebrates (4). Nup100p may have a role in recycling transport factors from the nucleus to the cytoplasm, as it interacts physically with Kap95p. The interaction involves the GLFG repeats of Nup100p and the nuclear export signal (NES) of Kap95p (19).

Last updated: 1999-08-06

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP100]

1) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
2) Strawn LA, et al.  (2001) The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem 276(9):6445-52
3) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
4) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
5) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
7) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
8) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
9) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
10) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
11) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
12) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
13) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
14) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
15) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
16) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
17) Ho AK, et al.  (1998) The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function. Mol Biol Cell 9(2):355-73
18) Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69
19) Iovine MK and Wente SR  (1997) A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol 137(4):797-811