NUP100/YKL068W Summary Help

Standard Name NUP100 1
Systematic Name YKL068W
Alias NSP100
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Also known as: [NUP100+] 11
Name Description NUclear Pore 12
Chromosomal Location
ChrXI:310199 to 313078 | ORF Map | GBrowse
Gene Ontology Annotations All NUP100 GO evidence and references
  View Computational GO annotations for NUP100
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
170 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 71
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Co-fractionation: 1
  • Co-purification: 1
  • PCA: 4
  • Reconstituted Complex: 12
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 30
  • Phenotypic Enhancement: 5
  • Positive Genetic: 7
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 959
Molecular Weight (Da) 99,988
Isoelectric Point (pI) 10.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:310199 to 313078 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2880 310199..313078 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001551

NUP100 encodes a nuclear pore protein (1, 13). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 13, 14, 15, 16). The structure of the vertebrate NPC has been studied extensively; recent reviews include 17, 18, 19, and 20. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (21, 22). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 13, 14, 23, 24, 25). NUP100 is not essential, but the nup100 deletion is synthetically lethal with mutations in genes encoding other nucleoporins and other nucleocytoplasmic transport factors (13, 26). Nup100p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (1, 13). Nup100p and Nup116p are similar over their entire lengths, and may have arisen by gene duplication (1, 27, 13). A related nucleoporin, Nup98, has been identified in vertebrates (13). Nup100p may have a role in recycling transport factors from the nucleus to the cytoplasm, as it interacts physically with Kap95p. The interaction involves the GLFG repeats of Nup100p and the nuclear export signal (NES) of Kap95p (28).

Last updated: 1999-08-06 Contact SGD

References cited on this page View Complete Literature Guide for NUP100
1) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
2) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
3) Strawn LA, et al.  (2001) The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem 276(9):6445-52
4) Pyhtila B and Rexach M  (2003) A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex. J Biol Chem 278(43):42699-709
5) Menon BB, et al.  (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
8) Light WH, et al.  (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25
9) Jovanovic-Talisman T, et al.  (2009) Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 457(7232):1023-7
10) Meinema AC, et al.  (2011) Long unfolded linkers facilitate membrane protein import through the nuclear pore complex. Science 333(6038):90-3
11) Halfmann R, et al.  (2012) Prion formation by a yeast GLFG nucleoporin. Prion 6(4):391-9
12) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
13) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
14) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
15) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
16) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
17) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
18) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
19) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
20) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
21) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
22) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
23) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
24) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
25) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
26) Ho AK, et al.  (1998) The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function. Mol Biol Cell 9(2):355-73
27) Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69
28) Iovine MK and Wente SR  (1997) A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol 137(4):797-811