NUP120/YKL057C Summary Help

NUP120 BASIC INFORMATION

Standard Name NUP120
Systematic Name YKL057C
Alias RAT2
Feature Type ORF, Verified
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p (1, 2, 3, 4 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP120 GO evidence and references
    View Computational GO annotations for NUP120
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP120 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions NUP120 All interactions details and references
222 total interaction(s) for 145 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-Western: 5
  • Co-localization: 1
  • Co-purification: 3
  • FRET: 5
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 69
  • Phenotypic Suppression: 24
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 51
Sequence Information
ChrXI:333613 to 330500 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2005-12-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3114 333613..330500 2005-12-15 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001540

NUP120 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP120

SUMMARY PARAGRAPH for NUP120

NUP120 encodes a non-essential nuclear pore protein (3, 2). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 5, 6, 7, 8). The structure of the vertebrate NPC has been studied extensively; recent reviews include 9, 10, 11, and 12. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (13, 14). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 5, 6, 15, 16, 17). Nup120p is part of subcomplex within the NPC that also includes Sec13p, Seh1p, Nup84p, and Nup85p (18, 5). PH, A nup120 mutation is synthetically lethal with mutations in NUP133 and NUP159, which also encode nuclear pore proteins. Also, Nup85p cannot be detected in nup120 mutants (19).

Last updated: 1999-08-09

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP120]

1) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
2) Heath CV, et al.  (1995) Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene. J Cell Biol 131(6 Pt 2):1677-97
3) Aitchison JD, et al.  (1995) Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J Cell Biol 131(6 Pt 2):1659-75
4) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
5) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
6) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
8) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
9) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
10) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
11) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
12) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
13) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
14) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
15) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
16) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
17) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
18) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
19) Goldstein AL, et al.  (1996) Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol Biol Cell 7(6):917-34