DEF1/YKL054C Summary Help

Standard Name DEF1 1
Systematic Name YKL054C
Alias VID31
Feature Type ORF, Verified
Description RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis (1, 2, 3)
Name Description RNAPII DEgradation Factor 1
Chromosomal Location
ChrXI:338753 to 336537 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All DEF1 GO evidence and references
  View Computational GO annotations for DEF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 112 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
92 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-purification: 2
  • PCA: 26
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 9
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 738
Molecular Weight (Da) 83,973
Isoelectric Point (pI) 4.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:338753 to 336537 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2217 338753..336537 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001537
References cited on this page View Complete Literature Guide for DEF1
1) Woudstra EC, et al.  (2002) A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage. Nature 415(6874):929-33
2) Somesh BP, et al.  (2005) Multiple mechanisms confining RNA polymerase II ubiquitylation to polymerases undergoing transcriptional arrest. Cell 121(6):913-23
3) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222