Standard Name	DEF1
Systematic Name	YKL054C
Alias	VID31
Feature Type	ORF, Verified
Description	RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis (1, 2, 3)
Name Description	RNAPII DEgradation Factor 1

Chromosomal Location	ChrXI:338753 to 336537 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for DEF1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	response to DNA damage stimulus (IMP) telomere maintenance (IMP) transcription-coupled nucleotide-excision repair (IGI) ubiquitin-dependent protein catabolic process (IDA, IMP)
Cellular Component
Manually curated	nucleus (IMP)

Classical genetics
null	X ray resistance: decreased glutathione accumulation: increased cytokinesis: decreased meiosis: abnormal metal resistance: decreased Ptr2p-GFP distribution: abnormal resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to 4-aminofolic acid: decreased
overexpression	glutathione accumulation: increased hydrogen sulfide excretion: increased
Large-scale survey
null	cell size: increased proton accumulation: decreased glycogen accumulation: increased polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate haploinsufficient heat sensitivity: increased nutrient utilization: increased resistance to 5-fluorouracil: decreased resistance to hydroxyurea: decreased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to methyl methanesulfonate: decreased resistance to nickel sulfate: decreased resistance to ethanol: decreased resistance to camptothecin: decreased resistance to benomyl: decreased resistance to caffeine: decreased resistance to sirolimus: decreased resistance to tunicamycin: decreased resistance to (S)-lactic acid: decreased resistance to acetic acid: decreased resistance to hydrogen chloride: decreased resistance to propolis extract: decreased resistance to 4-chlorophenol: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to edelfosine: decreased resistance to sorbate: decreased respiratory growth: absent sporulation: decreased stress resistance: decreased toxin resistance: decreased toxin resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	77 total interaction(s) for 66 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-RNA: 4 Affinity Capture-Western: 3 Biochemical Activity: 1 Co-purification: 2 PCA: 26 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Phenotypic Enhancement: 6 Phenotypic Suppression: 3 Synthetic Growth Defect: 5 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:338753 to 336537 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2217 338753..336537 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001537