ASK1/YKL052C Summary

ASK1 BASIC INFORMATION

Standard Name	ASK1 1
Systematic Name	YKL052C
Feature Type	ORF, Verified
Description	Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases (1, 2, 3, 4, 5)
Name Description	Associated with Spindles and Kinetochores 1

GO Annotations	All ASK1 GO evidence and references
	View Computational GO annotations for ASK1
Molecular Function
Manually curated	structural constituent of cytoskeleton (IDA)
Biological Process
Manually curated	mitotic spindle organization in nucleus (IMP) regulation of microtubule polymerization or depolymerization (IDA, IPI)
Cellular Component
Manually curated	condensed nuclear chromosome kinetochore (IDA) condensed nuclear chromosome, centromeric region (IDA) DASH complex (IDA, IPI) spindle (IDA)

Mutant Phenotype	All ASK1 Phenotype details and references
Classical genetics
conditional	cell cycle passage through the metaphase-anaphase transition: delayed resistance to hydroxyurea: decreased spindle morphology: abnormal
Large-scale survey
null	inviable
overexpression	cell cycle progression: abnormal
repressible	mitochondrial morphology: abnormal

Interactions	ASK1 All interactions details and references
	64 total interaction(s) for 23 unique genes/features.
Physical Interactions	Affinity Capture-MS: 20 Affinity Capture-Western: 12 Biochemical Activity: 2 Co-localization: 1 Co-purification: 7 Two-hybrid: 7
Genetic Interactions	Dosage Rescue: 5 Phenotypic Suppression: 1 Synthetic Growth Defect: 2 Synthetic Lethality: 5 Synthetic Rescue: 2

Sequence Information

ChrXI:340190 to 339312 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-12-15 | Sequence: 2008-06-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..879	340190..339312	2005-12-15	2008-06-03

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001535

ASK1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ASK1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ASK1]

1)	Li Y, et al. (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
2)	Li Y and Elledge SJ (2003) The DASH complex component Ask1 is a cell cycle-regulated Cdk substrate in Saccharomyces cerevisiae. Cell Cycle 2(2):143-8
3)	Miranda JJ, et al. (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4)	Westermann S, et al. (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5)	Westermann S, et al. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9

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