Standard Name	ASK1 1
Systematic Name	YKL052C
Feature Type	ORF, Verified
Description	Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description	Associated with Spindles and Kinetochores 1

Chromosomal Location	ChrXI:340546 to 339668 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for ASK1
Molecular Function
Manually curated	contributes_to microtubule binding (IDA) structural constituent of cytoskeleton (IDA)
Biological Process
Manually curated	attachment of spindle microtubules to kinetochore (IDA) attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation (IC) mitotic sister chromatid segregation (IMP) mitotic spindle organization in nucleus (IMP) regulation of microtubule polymerization or depolymerization (IDA, IPI)
Cellular Component
Manually curated	condensed nuclear chromosome kinetochore (IDA) condensed nuclear chromosome, centromeric region (IDA) DASH complex (IDA, IPI) mitotic spindle (IDA)

Classical genetics
conditional	cell cycle passage through the metaphase-anaphase transition: delayed resistance to hydroxyurea: decreased spindle morphology: abnormal
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal colony sectoring: increased
null	haploinsufficient inviable starvation resistance: decreased
overexpression	cell cycle progression: abnormal
reduction of function	competitive fitness: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	90 total interaction(s) for 36 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-RNA: 3 Affinity Capture-Western: 13 Biochemical Activity: 2 Co-crystal Structure: 1 Co-localization: 1 Co-purification: 17 PCA: 2 Reconstituted Complex: 2 Two-hybrid: 9
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 5 Phenotypic Suppression: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 6 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:340546 to 339668 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 2008-06-03
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..879 340546..339668 2011-02-03 2008-06-03
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001535