CSE4/YKL049C Summary Help

Standard Name CSE4 1
Systematic Name YKL049C
Alias CSL2 2
Feature Type ORF, Verified
Description Centromere protein that resembles histone H3; associated with promoters, accessible chromatin and RNA polymerase II-bound regions; phosphorylated Cse4p associates with centromeres; required for proper kinetochore function; levels regulated by E3 ubiquitin ligase Psh1p; phosphorylation of Cse4p may destabilize defective kinetochores to promote bi-orientation; ubiquitination of N terminus regulates Cse4p proteolysis for faithful chromosome segregation; human CENP-A homolog (1, 3, 4, 5, 6, 7, 8)
Name Description Chromosome SEgregation 1
Chromosomal Location
ChrXI:346764 to 346075 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All CSE4 GO evidence and references
  View Computational GO annotations for CSE4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
457 total interaction(s) for 352 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 314
  • Affinity Capture-Western: 36
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Co-localization: 10
  • FRET: 1
  • PCA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 10
  • Dosage Rescue: 12
  • Negative Genetic: 2
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 29
  • Synthetic Lethality: 16
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 229
Molecular Weight (Da) 26,841
Isoelectric Point (pI) 10.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:346764 to 346075 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2003-01-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..690 346764..346075 2011-02-03 2003-01-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001532
References cited on this page View Complete Literature Guide for CSE4
1) Stoler S, et al.  (1995) A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev 9(5):573-86
2) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3) Meluh PB, et al.  (1998) Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell 94(5):607-13
4) Ranjitkar P, et al.  (2010) An E3 Ubiquitin Ligase Prevents Ectopic Localization of the Centromeric Histone H3 Variant via the Centromere Targeting Domain. Mol Cell 40(3):455-64
5) Hewawasam G, et al.  (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
6) Lefrancois P, et al.  (2013) Centromere-like regions in the budding yeast genome. PLoS Genet 9(1):e1003209
7) Au WC, et al.  (2013) A novel role of the N terminus of budding yeast histone h3 variant cse4 in ubiquitin-mediated proteolysis. Genetics 194(2):513-8
8) Boeckmann L, et al.  (2013) Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Mol Biol Cell 24(12):2034-2044