VPS24/YKL041W Summary 
| Standard Name | VPS24 1, 2, 3 |
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| Systematic Name | YKL041W |
| Alias | DID3 4 , VPL26 5 |
| Feature Type | ORF, Verified |
| Description | One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway (1, 6, 7 and see Summary Paragraph) |
| Name Description | Vacuolar Protein Sorting 2, 3 |
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| View Computational GO annotations for VPS24 | |
| Molecular Function | |
| Manually curated |
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| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| 184 total interaction(s) for 157 unique genes/features. | |
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| Genetic Interactions |
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| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
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| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000001524 |
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Most proteins targeted for degradation in the multivesicular body (MVB) pathway are monoubiquitinated, then passed through the series of highly-conserved multisubunit complexes known as ESCRT (Endosomal Sorting Complex Required for Transport). ESCRT I, II, and III are sequentially mobilized to endosomal membranes, where they direct protein sorting and MVB biogenesis, and play a crucial role in retrovirus budding (8).
The formation of ESCRT III - composed of subcomplexes Snf7p-Vps20p and Did4p-Vps24p - is transient and appears to be dependent on ESCRT II (9, 10). Snf7p-Vps20p effects the membrane association of ESCRT III, with Snf7p being required for localization of the Bro1p vacuolar protein sorting factor to endosomes (11, 12), and Vps20p for formation of ESCRT III (13, 10). The Did4p-Vps24p subcomplex is required for endosomal localization of the Doa4p ubiquitin hydrolase, as well as the Vps4p AAA-type ATPase, which is necessary for the dissociation of ESCRT III (10, 4, 9, 14, 7, 8).
vps24 null mutants are hypersensitive to high temperatures and to canavanine, and overproduction of Did4p suppresses both of these sensitivities (15, 4). vps24 null mutants also display hypersensitivity to caffeine and rapamycin (16). cnb1 vps24 double null mutants are synthetically sick (16), and vps24 null mutants display synthetic sickness when combined with overproduction of alpha-synuclein (human SNCA), which is the major component of the inclusion bodies associated with Parkinson's disease (17).
Last updated: 2006-03-10 
| 1) | Babst M, et al. (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93 |
| 2) | Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48 |
| 3) | Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65 |
| 4) | Amerik AY, et al. (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80 |
| 5) | Raymond CK, et al. (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402 |
| 6) | Raiborg C, et al. (2003) Protein sorting into multivesicular endosomes. Curr Opin Cell Biol 15(4):446-55 |
| 7) | Bowers K, et al. (2004) Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5(3):194-210 |
| 8) | Teo H, et al. (2004) ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes. Dev Cell 7(4):559-69 |
| 9) | Babst M, et al. (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9 |
| 10) | Babst M, et al. (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82 |
| 11) | Odorizzi G, et al. (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116(Pt 10):1893-903 |
| 12) | Boysen JH and Mitchell AP (2006) Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway. Mol Biol Cell 17(3):1344-53 |
| 13) | Ashrafi K, et al. (1998) A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase. J Biol Chem 273(40):25864-74 |
| 14) | Yeo SC, et al. (2003) Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae. J Cell Sci 116(Pt 19):3957-70 |
| 15) | Eguez L, et al. (2004) Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting. Genetics 167(1):107-17 |
| 16) | Parsons AB, et al. (2004) Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways. Nat Biotechnol 22(1):62-9 |
| 17) | Willingham S, et al. (2003) Yeast genes that enhance the toxicity of a mutant huntingtin fragment or alpha-synuclein. Science 302(5651):1769-72 |
