CDC16/YKL022C Summary Help

Standard Name CDC16 1
Systematic Name YKL022C
Feature Type ORF, Verified
Description Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia (2, 3 and see Summary Paragraph)
Name Description Cell Division Cycle 4
Chromosomal Location
ChrXI:397248 to 394726 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -15 cM
Gene Ontology Annotations All CDC16 GO evidence and references
  View Computational GO annotations for CDC16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
reduction of function
237 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 126
  • Affinity Capture-Western: 74
  • Biochemical Activity: 3
  • Reconstituted Complex: 5
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 4
  • Dosage Rescue: 5
  • Negative Genetic: 1
  • Phenotypic Enhancement: 4
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 3
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 840
Molecular Weight (Da) 94,991
Isoelectric Point (pI) 6.74
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:397248 to 394726 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -15 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2523 397248..394726 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001505

CDC16 (APC6 (5)) is an essential member of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (2). This protein contains tetratrico peptide repeats (TPR), a protein-protein interaction motif (6). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (7, 8, 9, 10, 11, 12). Cdc16p interacts with two other essential APC subunits, Cdc23p and Cdc27p (13). Several temperature-sensitive mutants of CDC16 arrest as large-budded cells with the nucleus at the neck and are defective in the ubiquitination of Clb2p at non-permissive temperature (12, 4). The cdc16-183 mutant displays a metaphase arrest at non-permissive temperature but is also super-sensitive to nocodazole (14). The human homologue, CDC16Hs, has been localized to the centrosomes and the mitotic spindle (15).

Many thanks to Patricia Melloy for writing this gene summary.

Last updated: 2004-11-15 Contact SGD

References cited on this page View Complete Literature Guide for CDC16
1) Hartwell LH, et al.  (1973) Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics 74(2):267-286
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
4) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
5) Yu H, et al.  (1998) Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279(5354):1219-22
6) Lamb JR, et al.  (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257-9
7) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
8) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
9) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
10) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
11) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
12) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
13) Lamb JR, et al.  (1994) Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. EMBO J 13(18):4321-8
14) Lai LA, et al.  (2003) A novel yeast mutant that is defective in regulation of the Anaphase-Promoting Complex by the spindle damage checkpoint. Mol Genet Genomics 270(2):156-64
15) Tugendreich S, et al.  (1995) CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell 81(2):261-8