CDC16/YKL022C Summary Help

CDC16 BASIC INFORMATION

Standard Name CDC16
Systematic Name YKL022C
Feature Type ORF, Verified
Description Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation (1 and see Summary Paragraph)
Name Description Cell Division Cycle 2
GO Annotations All CDC16 GO evidence and references
    View Computational GO annotations for CDC16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CDC16 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
overexpression
repressible
Interactions CDC16 All interactions details and references
181 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 111
  • Affinity Capture-Western: 46
  • Biochemical Activity: 3
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3
Sequence Information
ChrXI:396891 to 394369 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -15 cM
Last Update Coordinates: 2005-12-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2523 396891..394369 2005-12-15 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001505

CDC16 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CDC16

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
BUB2Nomenclature conflictCDC16/YKL022C encodes a subunit of the Anaphase Promoting Complex. Do not confuse CDC16/YKL022C with pombe cdc16, which is similar to the cerevisiae BUB2/YMR055C gene.

SUMMARY PARAGRAPH for CDC16

CDC16 (APC6 (3)) is an essential member of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (1). This protein contains tetratrico peptide repeats (TPR), a protein-protein interaction motif (4). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassembly factor, Ase1p (5, 6, 7, 8, 9, 10). Cdc16p interacts with two other essential APC subunits, Cdc23p and Cdc27p (11). Several temperature-sensitive mutants of CDC16 arrest as large-budded cells with the nucleus at the neck and are defective in the ubiquitination of Clb2p at non-permissive temperature (10, 2). The cdc16-183 mutant displays a metaphase arrest at non-permissive temperature but is also super-sensitive to nocodazole (12). The human homologue, CDC16Hs, has been localized to the centrosomes and the mitotic spindle (13).

Many thanks to Patricia Melloy for writing this gene summary.

Last updated: 2004-11-15

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC16]

1) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
2) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
3) Yu H, et al.  (1998) Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279(5354):1219-22
4) Lamb JR, et al.  (1995) Tetratrico peptide repeat interactions: to TPR or not to TPR? Trends Biochem Sci 20(7):257-9
5) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
6) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
7) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
8) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
9) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
10) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
11) Lamb JR, et al.  (1994) Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis. EMBO J 13(18):4321-8
12) Lai LA, et al.  (2003) A novel yeast mutant that is defective in regulation of the Anaphase-Promoting Complex by the spindle damage checkpoint. Mol Genet Genomics 270(2):156-64
13) Tugendreich S, et al.  (1995) CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell 81(2):261-8