SPT23/YKL020C Summary

SPT23 BASIC INFORMATION

Standard Name	SPT23
Systematic Name	YKL020C
Feature Type	ORF, Verified
Description	ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting (1, 2)
Name Description	SuPpressor of Ty

GO Annotations	All SPT23 GO evidence and references
	View Computational GO annotations for SPT23
Molecular Function
Manually curated	transcription activator activity (IMP)
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	fatty acid metabolic process (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) response to cold (IMP)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IDA, TAS) nucleus (IC)

Regulatory Role
Binding motifs	SPT23 Transcription Factor Binding Sites and References
	RAAATSAA

Mutant Phenotype	All SPT23 Phenotype details and references
Classical genetics
overexpression	budding: abnormal cell size: abnormal cellular morphology: abnormal
Large-scale survey
null	resistance to PM02734: increased viable
overexpression	vegetative growth: decreased

Interactions	SPT23 All interactions details and references
	72 total interaction(s) for 47 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Affinity Capture-Western: 9 Biochemical Activity: 3 Reconstituted Complex: 2 Two-hybrid: 4
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 28 Phenotypic Suppression: 14 Synthetic Lethality: 4 Synthetic Rescue: 4

Sequence Information

ChrXI:401723 to 398475 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-12-15 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3249	401723..398475	2005-12-15	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001503

SPT23 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SPT23

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPT23]

1)	Chellappa R, et al. (2001) The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem 276(47):43548-56
2)	Rape M, et al. (2001) Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell 107(5):667-77

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