SPT23/YKL020C Summary Help

Standard Name SPT23
Systematic Name YKL020C
Feature Type ORF, Verified
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication (1, 2, 3)
Name Description SuPpressor of Ty
Chromosomal Location
ChrXI:402080 to 398832 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPT23 GO evidence and references
Molecular Function
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
164 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 17
  • Biochemical Activity: 7
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 78
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 3
  • Positive Genetic: 20
  • Synthetic Lethality: 6
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,082
Molecular Weight (Da) 121,337
Isoelectric Point (pI) 6.65
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:402080 to 398832 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3249 402080..398832 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001503
References cited on this page View Complete Literature Guide for SPT23
1) Chellappa R, et al.  (2001) The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem 276(47):43548-56
2) Rape M, et al.  (2001) Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell 107(5):667-77
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104