SWD2/YKL018W Summary Help

Standard Name SWD2 1
Systematic Name YKL018W
Alias CPS35 2 , SAF37 3
Feature Type ORF, Verified
Description Subunit of the COMPASS (Set1C) histone H3K4 methyltransferase complex; required for Set1C stability and optimal activity; COMPASS methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Set1c, WD40 repeat protein
Chromosomal Location
ChrXI:404102 to 405091 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SWD2 GO evidence and references
  View Computational GO annotations for SWD2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
conditional
null
overexpression
Resources
182 total interaction(s) for 75 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 115
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Co-purification: 3
  • Protein-peptide: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 32
  • Phenotypic Suppression: 3
  • Positive Genetic: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 329
Molecular Weight (Da) 37,122
Isoelectric Point (pI) 5.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:404102 to 405091 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..990 404102..405091 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001501
References cited on this page View Complete Literature Guide for SWD2
1) Roguev A, et al.  (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J 20(24):7137-48
2) Miller T, et al.  (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci U S A 98(23):12902-7
3) Nagy PL, et al.  (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99(1):90-4
4) Krogan NJ, et al.  (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277(13):10753-5
5) Sun ZW and Allis CD  (2002) Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast. Nature 418(6893):104-8
6) Dover J, et al.  (2002) Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem 277(32):28368-71
7) Cheng H, et al.  (2004) The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3. Mol Cell Biol 24(7):2932-43
8) Nedea E, et al.  (2008) The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes. Mol Cell 29(5):577-87