AUR1/YKL004W Summary

Standard Name	AUR1 1, 2
Systematic Name	YKL004W
Feature Type	ORF, Verified
Description	Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance (2, 3 and see Summary Paragraph)
Name Description	AUreobasidin A Resistance 1, 2

Chromosomal Location	ChrXI:435580 to 436785 \| ORF Map \| GBrowse

Gene Ontology Annotations All AUR1 GO evidence and references

	View Computational GO annotations for AUR1
Molecular Function
Manually curated	inositol phosphoceramide synthase activity (IDA, IMP)
Biological Process
Manually curated	inositolphosphoceramide metabolic process (IDA) sphingolipid biosynthetic process (IMP)
Cellular Component
Manually curated	Golgi apparatus (IDA) inositol phosphoceramide synthase complex (IPI)
High-throughput	integral to membrane (ISM)

Pathways	sphingolipid metabolism

Mutant phenotypes All AUR1 Phenotype evidence and references

Classical genetics
conditional	inositol phosphoceramide accumulation: decreased ceramide accumulation: increased
null	inviable
overexpression	hyperosmotic stress resistance: increased resistance to Aureobasidin A: increased
repressible	chitin deposition: abnormal cytoskeleton morphology: abnormal beta-tubulin accumulation: decreased
unspecified	resistance to Aureobasidin A: increased
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All AUR1 Interaction evidence and references

	212 total interaction(s) for 172 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 3 PCA: 17 Protein-RNA: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 154 Phenotypic Enhancement: 2 Positive Genetic: 23 Synthetic Growth Defect: 1 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All AUR1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:435580 to 436785 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1206	435580..436785	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001487

SUMMARY PARAGRAPH for AUR1

About sphingolipid metabolism

Sphingolipids are essential components of the plasma membrane in all eukaryotic cells. S. cerevisiae cells make three complex sphingolipids: inositol-phosphoceramide (IPC), mannose-inositol-phosphoceramide (MIPC), and mannose-(inositol phosphate)2-ceramide (M(IP)2C)(4). In the yeast plasma membrane sphingolipids concentrate with ergosterol to form lipid rafts, specialized membrane microdomains implicated in a variety of cellular processes, including sorting of membrane proteins and lipids, as well as organizing and regulating signaling cascades (5). Intermediates in sphingolipid biosynthesis have been shown to play important roles as signaling molecules and growth regulators. Sphingolipid long chain bases (LCBs), dihydrosphingosine (DHS) and phytosphingosine (PHS), have been implicated as secondary messengers in signaling pathways that regulate the heat stress response (6, 7). Other intermediates, phytoceramide and long-chain base phosphates (LCBPs), have been shown to be components of the tightly-controlled ceramide/LCBP rheostat, which regulates cell growth (8). Since phosphoinositol-containing sphingolipids are unique to fungi, the sphingolipid biosynthesis pathway is considered a target for antifungal drugs (3, 9).

Last updated: 2007-10-05

References cited on this page View Complete Literature Guide for AUR1

1)	Hashida-Okado T, et al. (1996) AUR1, a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae: a study of defective morphologies in Aur1p-depleted cells. Mol Gen Genet 251(2):236-44
2)	Heidler SA and Radding JA (1995) The AUR1 gene in Saccharomyces cerevisiae encodes dominant resistance to the antifungal agent aureobasidin A (LY295337). Antimicrob Agents Chemother 39(12):2765-9
3)	Nagiec MM, et al. (1997) Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene. J Biol Chem 272(15):9809-17
4)	Dickson RC and Lester RL (2002) Sphingolipid functions in Saccharomyces cerevisiae. Biochim Biophys Acta 1583(1):13-25
5)	Bagnat M and Simons K (2002) Lipid rafts in protein sorting and cell polarity in budding yeast Saccharomyces cerevisiae. Biol Chem 383(10):1475-80
6)	Jenkins GM, et al. (1997) Involvement of yeast sphingolipids in the heat stress response of Saccharomyces cerevisiae. J Biol Chem 272(51):32566-72
7)	Ferguson-Yankey SR, et al. (2002) Mutant analysis reveals complex regulation of sphingolipid long chain base phosphates and long chain bases during heat stress in yeast. Yeast 19(7):573-86
8)	Kobayashi SD and Nagiec MM (2003) Ceramide/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p. Eukaryot Cell 2(2):284-94
9)	Sugimoto Y, et al. (2004) IPC synthase as a useful target for antifungal drugs. Curr Drug Targets Infect Disord 4(4):311-22