MGA2/YIR033W Summary

MGA2 BASIC INFORMATION

Standard Name	MGA2
Systematic Name	YIR033W
Feature Type	ORF, Verified
Description	ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting (1, 2)

GO Annotations	All MGA2 GO evidence and references
	View Computational GO annotations for MGA2
Molecular Function
Manually curated	transcription activator activity (IMP)
Biological Process
Manually curated	fatty acid metabolic process (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) response to cold (IMP)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IDA)

Mutant Phenotype	All MGA2 Phenotype details and references
Classical genetics
null	metal resistance: decreased
Large-scale survey
null	alkaline pH resistance: decreased competitive fitness: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to St. John's Wort extract: decreased viable

Interactions	MGA2 All interactions details and references
	140 total interaction(s) for 94 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Biochemical Activity: 2 Reconstituted Complex: 3 Two-hybrid: 21
Genetic Interactions	Dosage Rescue: 3 Phenotypic Enhancement: 50 Phenotypic Suppression: 33 Synthetic Growth Defect: 11 Synthetic Lethality: 7 Synthetic Rescue: 1

Sequence Information

ChrIX:416121 to 419462 | |

Last Update

Coordinates: 1994-12-10 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3342	416121..419462	1994-12-10	1994-12-10

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001472

MGA2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for MGA2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MGA2]

1)	Chellappa R, et al. (2001) The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem 276(47):43548-56
2)	Shcherbik N, et al. (2003) Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90. Curr Biol 13(14):1227-33

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