DAL3/YIR032C Summary

Standard Name	DAL3 1
Systematic Name	YIR032C
Feature Type	ORF, Verified
Description	Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression (2, 3 and see Summary Paragraph)
Name Description	Degradation of Allantoin

Chromosomal Location	ChrIX:415617 to 415030 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 37 cM

Gene Ontology Annotations All DAL3 GO evidence and references

	View Computational GO annotations for DAL3
Molecular Function
Manually curated	ureidoglycolate hydrolase activity (IMP)
Biological Process
Manually curated	allantoin catabolic process (IC)
Cellular Component
Manually curated	membrane (ISS)

Pathways	allantoin degradation

Mutant phenotypes All DAL3 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	competitive fitness: increased resistance to selenomethionine: increased resistance to tirapazamine: increased resistance to cycloheximide: decreased resistance to paromomycin: decreased resistance to neomycin: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All DAL3 Interaction evidence and references

	18 total interaction(s) for 16 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Protein-RNA: 1 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 5 Positive Genetic: 6 Synthetic Growth Defect: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All DAL3 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:415617 to 415030 | |

Note: this feature is encoded on the Crick strand.

: 37 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..588	415617..415030	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001471

SUMMARY PARAGRAPH for DAL3

About allantoin degradation

The allantoin degradation pathway, which converts allantoin to ammonia and carbon dioxide, allows S. cerevisiae to use allantoin as a sole nitrogen source. Conversion of allantoin to ammonia is carried out by the DAL1, DAL2, and DAL3 gene products, which work sequentially to generate urea (1). Urea is then degraded to ammonia in a two-step process by the DUR1,2 protein, a multifunctional single enzyme originally thought to be encoded by two tightly-linked genes (hence the name DUR1,2) (4). The allantoin catabolic pathway genes are regulated by a general signal that responds to the availability of readily utilizable nitrogen sources, and also by pathway-specific induction by allantoin or the intermediate allophanate. These regulatory effects are mediated by cis-acting DNA elements and the trans-acting factors Gln3p, Gat1p, Dal80p, Dal81p, and Dal82p (5, 6, 7, 8).

Last updated: 2007-10-03

References cited on this page View Complete Literature Guide for DAL3

1)	Yoo HS, et al. (1985) Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol 5(9):2279-88
2)	Yoo HS and Cooper TG (1991) The ureidoglycollate hydrolase (DAL3) gene in Saccharomyces cerevisiae. Yeast 7(7):693-8
3)	Magasanik B (1992) "Regulation of Nitrogen Utilization." Pp. 283-317 in the Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression,
4)	Cooper TG, et al. (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80
5)	Rai R, et al. (1999) Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. J Biol Chem 274(39):28026-34
6)	Magasanik B and Kaiser CA (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18
7)	Scott S, et al. (2000) Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. J Biol Chem 275(10):7198-204
8)	Talibi D, et al. (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7