DAL2/YIR029W Summary Help

Standard Name DAL2 1
Systematic Name YIR029W
Alias ALC1
Feature Type ORF, Verified
Description Allantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation (2, 3, 4 and see Summary Paragraph)
Name Description Degradation of Allantoin
Chromosomal Location
ChrIX:410807 to 411838 | ORF Map | GBrowse
Gbrowse
Genetic position: 36.1 cM
Gene Ontology Annotations All DAL2 GO evidence and references
  View Computational GO annotations for DAL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Pathways
Classical genetics
unspecified
Large-scale survey
null
Resources
20 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Co-crystal Structure: 1

Genetic Interactions
  • Negative Genetic: 11
  • Positive Genetic: 6
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 343
Molecular Weight (Da) 38,714
Isoelectric Point (pI) 6.33
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:410807 to 411838 | ORF Map | GBrowse
SGD ORF map
Genetic position: 36.1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1032 410807..411838 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001468
SUMMARY PARAGRAPH for DAL2

About allantoin degradation

The allantoin degradation pathway, which converts allantoin to ammonia and carbon dioxide, allows S. cerevisiae to use allantoin as a sole nitrogen source. Conversion of allantoin to ammonia is carried out by the DAL1, DAL2, and DAL3 gene products, which work sequentially to generate urea (1). Urea is then degraded to ammonia in a two-step process by the DUR1,2 protein, a multifunctional single enzyme originally thought to be encoded by two tightly-linked genes (hence the name DUR1,2) (5). The allantoin catabolic pathway genes are regulated by a general signal that responds to the availability of readily utilizable nitrogen sources, and also by pathway-specific induction by allantoin or the intermediate allophanate. These regulatory effects are mediated by cis-acting DNA elements and the trans-acting factors Gln3p, Gat1p, Dal80p, Dal81p, and Dal82p (6, 7, 8, 9).

Last updated: 2007-10-03 Contact SGD

References cited on this page View Complete Literature Guide for DAL2
1) Yoo HS, et al.  (1985) Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol 5(9):2279-88
2) Dorrington RA and Cooper TG  (1993) The DAL82 protein of Saccharomyces cerevisiae binds to the DAL upstream induction sequence (UIS). Nucleic Acids Res 21(16):3777-84
3) Yoo HS and Cooper TG  (1991) Sequences of two adjacent genes, one (DAL2) encoding allantoicase and another (DCG1) sensitive to nitrogen-catabolite repression in Saccharomyces cerevisiae. Gene 104(1):55-62
4) Magasanik B  (1992) "Regulation of Nitrogen Utilization." Pp. 283-317 in the Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Cooper TG, et al.  (1980) Structural analysis of the dur loci in S. cerevisiae: two domains of a single multifunctional gene. Genetics 94(3):555-80
6) Rai R, et al.  (1999) Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. J Biol Chem 274(39):28026-34
7) Magasanik B and Kaiser CA  (2002) Nitrogen regulation in Saccharomyces cerevisiae. Gene 290(1-2):1-18
8) Scott S, et al.  (2000) Functional domain mapping and subcellular distribution of Dal82p in Saccharomyces cerevisiae. J Biol Chem 275(10):7198-204
9) Talibi D, et al.  (1995) Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Nucleic Acids Res 23(4):550-7