MPH1/YIR002C Summary Help

Standard Name MPH1 1
Systematic Name YIR002C
Feature Type ORF, Verified
Description 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA in error-free bypass pathway, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; similar to FANCM human Fanconi anemia complementation group protein that with MHF complex is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases (2, 3, 4, 5, 6, 7, 8, 9)
Name Description Mutator PHenotype 1
Chromosomal Location
ChrIX:360396 to 357415 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MPH1 GO evidence and references
  View Computational GO annotations for MPH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
reduction of function
Large-scale survey
168 total interaction(s) for 92 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-Western: 8
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 4
  • Dosage Rescue: 4
  • Negative Genetic: 36
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 12
  • Positive Genetic: 4
  • Synthetic Growth Defect: 27
  • Synthetic Lethality: 2
  • Synthetic Rescue: 21

Expression Summary
Length (a.a.) 993
Molecular Weight (Da) 114,057
Isoelectric Point (pI) 7.27
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIX:360396 to 357415 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2982 360396..357415 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001441
References cited on this page View Complete Literature Guide for MPH1
1) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2) Shiratori A, et al.  (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53
3) Scheller J, et al.  (2000) MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage. Genetics 155(3):1069-81
4) Schurer KA, et al.  (2004) Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics 166(4):1673-86
5) Meetei AR, et al.  (2005) A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M. Nat Genet 37(9):958-63
6) Kang YH, et al.  (2009) The MPH1 Gene of Saccharomyces cerevisiae Functions in Okazaki Fragment Processing. J Biol Chem 284(16):10376-86
7) Yan Z, et al.  (2010) A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability. Mol Cell 37(6):865-78
8) Ward TA, et al.  (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884
9) Mazon G and Symington LS  (2013) Mph1 and mus81-mms4 prevent aberrant processing of mitotic recombination intermediates. Mol Cell 52(1):63-74