ATG32/YIL146C Summary Help

Standard Name ATG32 1, 2
Systematic Name YIL146C
Alias ECM37 3
Feature Type ORF, Verified
Description Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane; regulates mitophagy and ethanol production during alcoholic fermentation (1, 2, 4, 5)
Name Description AuTophaGy related 6
Chromosomal Location
ChrIX:75773 to 74184 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ATG32 GO evidence and references
  View Computational GO annotations for ATG32
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
65 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-crystal Structure: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 39
  • Positive Genetic: 3
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 529
Molecular Weight (Da) 58,968
Isoelectric Point (pI) 7.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:75773 to 74184 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1590 75773..74184 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001408
References cited on this page View Complete Literature Guide for ATG32
1) Kanki T, et al.  (2009) Atg32 is a mitochondrial protein that confers selectivity during mitophagy. Dev Cell 17(1):98-109
2) Okamoto K, et al.  (2009) Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy. Dev Cell 17(1):87-97
3) Lussier M, et al.  (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
4) Kondo-Okamoto N, et al.  (2012) Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy. J Biol Chem 287(13):10631-8
5) Shiroma S, et al.  (2014) Enhancement of Ethanol Fermentation in Saccharomyces cerevisiae Sake Yeast by Disrupting Mitophagy Function. Appl Environ Microbiol 80(3):1002-12
6) Klionsky DJ, et al.  (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45