STH1/YIL126W Summary

STH1/YIL126W Summary

Standard Name	STH1 1
Systematic Name	YIL126W
Alias	NPS1 2
Feature Type	ORF, Verified
Description	ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p (1, 3, 4, 5)
Name Description	SNF Two Homolog 1

Chromosomal Location	ChrIX:117992 to 122071 \| ORF Map \| GBrowse

	Genetic position: -91 cM

Gene Ontology Annotations All STH1 GO evidence and references

	View Computational GO annotations for STH1
Molecular Function
Manually curated	DNA translocase activity (IDA) DNA-dependent ATPase activity (IDA) helicase activity (IDA) histone acetyl-lysine binding (IDA)
Biological Process
Manually curated	ATP-dependent chromatin remodeling (IDA) chromatin remodeling at centromere (IMP) chromosome segregation (IGI) cytoskeleton organization (IGI, IMP) double-strand break repair (IMP) G2/M transition of mitotic cell cycle (IMP) meiosis (IMP) nucleosome disassembly (IDA) nucleosome positioning (IMP) regulation of transcription, DNA-dependent (IMP) transcription elongation from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	nucleus (IDA) RSC complex (IDA)

Mutant phenotypes All STH1 Phenotype evidence and references

Classical genetics
conditional	Ame1p-GFP distribution: abnormal Spc24p-GFP distribution: abnormal
null	inviable
repressible	cell cycle progression: arrested Pmp2GFP-KKTN distribution: decreased
Large-scale survey
conditional	resistance to benomyl: decreased
null	competitive fitness: decreased haploinsufficient heat sensitivity: increased inviable resistance to sulfanilamide: decreased
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to sirolimus: decreased
repressible	Pmp2GFP-LRKR distribution: decreased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All STH1 Interaction evidence and references

	249 total interaction(s) for 89 unique genes/features.
Physical Interactions	Affinity Capture-MS: 116 Affinity Capture-RNA: 2 Affinity Capture-Western: 46 Co-fractionation: 1 Co-purification: 13 Far Western: 3 Reconstituted Complex: 9 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 19 Negative Genetic: 3 Phenotypic Enhancement: 1 Phenotypic Suppression: 4 Synthetic Growth Defect: 17 Synthetic Lethality: 8 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All STH1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:117992 to 122071 | |

SGD ORF map

: -91 cM

Last Update

Coordinates: 1994-12-10 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4080	117992..122071	1994-12-10	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001388

References cited on this page View Complete Literature Guide for STH1

1)	Laurent BC, et al. (1992) An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family. Mol Cell Biol 12(4):1893-902
2)	Tsuchiya E, et al. (1992) The Saccharomyces cerevisiae NPS1 gene, a novel CDC gene which encodes a 160 kDa nuclear protein involved in G2 phase control. EMBO J 11(11):4017-26
3)	Yukawa M, et al. (1999) Nps1/Sth1p, a component of an essential chromatin-remodeling complex of Saccharomyces cerevisiae, is required for the maximal expression of early meiotic genes. Genes Cells 4(2):99-110
4)	Du J, et al. (1998) Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p homolog, is an essential ATPase in RSC and differs from Snf/Swi in its interactions with histones and chromatin-associated proteins. Genetics 150(3):987-1005
5)	Hsu JM, et al. (2003) The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. Mol Cell Biol 23(9):3202-15