HIS5/YIL116W Summary Help

Standard Name HIS5 1
Systematic Name YIL116W
Feature Type ORF, Verified
Description Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts (1, 2, 3, 4 and see Summary Paragraph)
Name Description HIStidine requiring 1
Chromosomal Location
ChrIX:142928 to 144085 | ORF Map | GBrowse
Gbrowse
Genetic position: -89 cM
Gene Ontology Annotations All HIS5 GO evidence and references
  View Computational GO annotations for HIS5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 19 genes
Resources
Pathways
Classical genetics
unspecified
Large-scale survey
null
Resources
11 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 5
  • Biochemical Activity: 1

Genetic Interactions
  • Negative Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 385
Molecular Weight (Da) 42,646
Isoelectric Point (pI) 6.37
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:142928 to 144085 | ORF Map | GBrowse
SGD ORF map
Genetic position: -89 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1158 142928..144085 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001378
SUMMARY PARAGRAPH for HIS5

HIS5 encodes histidinol-phosphate aminotransferase, which catalyzes the seventh step in histidine biosynthesis (3). The biosynthesis of histidine has been most extensively studied in Salmonella typhimurium and E. coli. The reactions and enzymes involved in histidine biosynthesis have been identified in many organisms, and are thoroughly reviewed in Alifano et al. (3). Mutations in HIS5, as well as in genes encoding other histidine biosynthetic enzymes, cause histidine auxotrophy and sensitivity to copper, cobalt, and nickel salts (2). Transcription of HIS5 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role (reviewed in 5).

Last updated: 1999-11-12 Contact SGD

References cited on this page View Complete Literature Guide for HIS5
1) Harashima S, et al.  (1981) Cloning of the HIS5 gene of Saccharomyces cerevisiae by yeast transformation. Gene 16(1-3):335-41
2) Pearce DA and Sherman F  (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9
3) Alifano P, et al.  (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69
4) Fink GR  (1964) GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. Science 146(3643):525-7
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press