COX5B/YIL111W Summary Help

Standard Name COX5B
Systematic Name YIL111W
Feature Type ORF, Verified
Description Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication (1, 2 and see Summary Paragraph)
Name Description Cytochrome c OXidase
Chromosomal Location
ChrIX:155222 to 155765 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All COX5B GO evidence and references
  View Computational GO annotations for COX5B
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
24 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Co-fractionation: 1
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 12
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 151
Molecular Weight (Da) 17,197
Isoelectric Point (pI) 10.62
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:155222 to 155765 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1 155222..155222 2011-02-03 1994-12-10
Intron 2..89 155223..155310 2011-02-03 1994-12-10
CDS 90..544 155311..155765 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001373
SUMMARY PARAGRAPH for COX5B

Cytochrome c oxidase catalyzes the terminal step in the electron transport chain involved in cellular respiration. This multisubunit enzyme of the mitochondrial inner membrane, also known as Complex IV, is composed of three core subunits encoded by the mitochondrial genome (Cox1p, Cox2p, and Cox3p) and eight additional subunits encoded by nuclear genes (Cox4p, Cox5Ap or Cox5Bp, Cox6p, Cox7p, Cox8p, Cox9p, Cox12p, and Cox13p) (3, 4, 5). The phenotype of a mutation affecting any of the genes encoding cytochrome c oxidase subunits, or any of the multiple genes required for expression or assembly of the subunits (reviewed in 6), is a decrease or block in respiratory growth. The inability to respire is not lethal since S. cerevisiae can grow by fermentation, but nonrespiring cells grow more slowly than respiratory-competent cells even on glucose-containing medium, resulting in smaller colony size. Such mutations causing "petite" colonies were first described by Ephrussi and colleagues around 1950 (7).

Last updated: 2006-08-18 Contact SGD

References cited on this page View Complete Literature Guide for COX5B
1) Hodge MR, et al.  (1989) Inverse regulation of the yeast COX5 genes by oxygen and heme. Mol Cell Biol 9(5):1958-64
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Taanman JW and Capaldi RA  (1992) Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J Biol Chem 267(31):22481-5
4) Geier BM, et al.  (1995) Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method. Eur J Biochem 227(1-2):296-302
5) Cooper CE, et al.  (1991) Cytochrome c oxidase: structure, function, and membrane topology of the polypeptide subunits. Biochem Cell Biol 69(9):586-607
6) Herrmann JM and Funes S  (2005) Biogenesis of cytochrome oxidase-sophisticated assembly lines in the mitochondrial inner membrane. Gene 354():43-52
7) Ephrussi B and Slonimski PP  (1955) Subcellular units involved in the synthesis of respiratory enzymes in yeast. Nature 176(4495):1207-8